Linked Life Data

16911584

Source:http://linkedlifedata.com/resource/pubmed/id/16911584

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2006-9-1
pubmed:abstractText
Brain tumor growth and progression is dependent upon vascularity, and is associated with altered ganglioside composition and distribution. In this study, we examined the influence of gangliosides on growth and vascularity in a malignant mouse astrocytoma, CT-2A. Ganglioside distribution was altered in CT-2A tumor cells using an antisense construct to beta-1,4-N-acetylgalactosaminyltransferase (GalNAc-T), a key enzyme that uses the simple ganglioside GM3 as a substrate for the synthesis of the more complex gangliosides, GM2, GM1 and GD1a. GalNAc-T gene expression was significantly lower in CT-2A cells stably transfected with the antisense GalNAc-T plasmid, pcDNA3.1/TNG (CT-2A/TNG) than in either non-transfected CT-2A or mock-transfected (CT-2A/V) control tumor cells. GM3 was elevated from 16% to 58% of the total ganglioside distribution, whereas GM1 and GD1a were reduced from 17% and 49% to 10% and 17%, respectively, in CT-2A/TNG tumor cells. Growth, vascularity (blood vessel density and Matrigel assay) and vascular endothelial growth factor (VEGF) expression was significantly less in CT-2A/TNG tumors than in control CT-2A brain tumors. In addition, the expression of VEGF, hypoxia-inducible factor 1alpha (HIF-1alpha) and neuropilin-1 (NP-1) was significantly lower in CT-2A/TNG tumor cells than in control CT-2A tumor cells. These data suggest that gene-linked changes in ganglioside composition influence the growth and angiogenic properties of the CT-2A astrocytoma.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1973-84
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:16911584-Animals, pubmed-meshheading:16911584-Astrocytoma, pubmed-meshheading:16911584-Brain Neoplasms, pubmed-meshheading:16911584-Cell Proliferation, pubmed-meshheading:16911584-Down-Regulation, pubmed-meshheading:16911584-Gangliosides, pubmed-meshheading:16911584-Hypoxia-Inducible Factor 1, alpha Subunit, pubmed-meshheading:16911584-Mice, pubmed-meshheading:16911584-Mice, Inbred C57BL, pubmed-meshheading:16911584-Mice, SCID, pubmed-meshheading:16911584-N-Acetylgalactosaminyltransferases, pubmed-meshheading:16911584-Neuropilin-1, pubmed-meshheading:16911584-Oligonucleotides, Antisense, pubmed-meshheading:16911584-Plasmids, pubmed-meshheading:16911584-Tissue Distribution, pubmed-meshheading:16911584-Transfection, pubmed-meshheading:16911584-Vascular Endothelial Growth Factor A
pubmed:year
2006
pubmed:articleTitle
Gene-linked shift in ganglioside distribution influences growth and vascularity in a mouse astrocytoma.
pubmed:affiliation
Department of Biology, Boston College, Chestnut Hill, Massachusetts, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural