Linked Life Data

1690673

Source:http://linkedlifedata.com/resource/pubmed/id/1690673

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-5-2
pubmed:abstractText
Certain types of glial structures, located at strategic positions along axon pathways, may provide the mechanical and/or chemical elements for the construction of barriers which can grossly direct the elongation of axons during development. The roof plate, a putative axon barrier, is located along the dorsal midline of the developing spinal cord and may be important for the guidance of the commissural and dorsal column axons. We examined the roof plate to determine the developmental morphology of the region and to determine which molecules were correlated with the barrier function when axons were growing nearby. Light and electron microscopic observations of the roof plate revealed that this glial domain undergoes a dramatic change in shape from a "wedge" with large extracellular spaces between the cell apices at E12.5 to a thin, dense septum with reduced extracellular space at E15.5. Immunocytochemical techniques demonstrated that highly sialylated neural cell adhesion molecule (N-CAM), the carbohydrate recognized by L2 monoclonal antibody, cholinesterase, stage-specific embryonic antigen 1, and a ligand that binds tetragonolobus purpureas agglutinin are expressed by the roof plate. These molecules, however, were also found in other regions of the spinal cord which are permissive or attractive to axon growth. A molecule which is unique to the roof plate when axons grow close to, but do not cross, the dorsal midline is a glycosaminoglycan (GAG), keratan sulfate. Keratan sulfate is also present in the tectal midline and in other noninnervated regions such as the outer epidermis and developing cartilage. Our data suggest that keratan sulfate, alone or in combination with other molecules expressed by the roof plate, may be responsible, in part, for the inhibition of axon elongation through the roof plate in the embryonic spinal cord.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0012-1606
pubmed:author
pubmed:issnType
Print
pubmed:volume
138
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
359-76
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:1690673-Animals, pubmed-meshheading:1690673-Animals, Newborn, pubmed-meshheading:1690673-Antibodies, Monoclonal, pubmed-meshheading:1690673-Antigens, pubmed-meshheading:1690673-Antigens, CD15, pubmed-meshheading:1690673-Axons, pubmed-meshheading:1690673-Cell Adhesion Molecules, Neuronal, pubmed-meshheading:1690673-Cholinesterases, pubmed-meshheading:1690673-Cricetinae, pubmed-meshheading:1690673-Embryonic and Fetal Development, pubmed-meshheading:1690673-Epitopes, pubmed-meshheading:1690673-Glycolipids, pubmed-meshheading:1690673-Keratan Sulfate, pubmed-meshheading:1690673-Mesocricetus, pubmed-meshheading:1690673-Microscopy, Electron, pubmed-meshheading:1690673-Neuroglia, pubmed-meshheading:1690673-Rats, pubmed-meshheading:1690673-Rats, Inbred Strains, pubmed-meshheading:1690673-Spinal Cord, pubmed-meshheading:1690673-Superior Colliculi
pubmed:year
1990
pubmed:articleTitle
Molecular and cellular characterization of the glial roof plate of the spinal cord and optic tectum: a possible role for a proteoglycan in the development of an axon barrier.
pubmed:affiliation
Center for Neurosciences, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't