Linked Life Data

1690189

Source:http://linkedlifedata.com/resource/pubmed/id/1690189

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1990-4-13
pubmed:abstractText
The immunocytochemical visualization of the peroxisomal beta-oxidation enzymes was investigated in three human post mortem liver samples. Acyl-CoA oxidase, bifunctional protein and 3-oxoacyl-CoA thiolase remained immunocytochemically detectable 30, 55 and 72 h after death. Peroxisomes in the parenchymal cells were clearly visualized for light microscopy (paraffin and cryostat sections), using protein A-gold in combination with silver enhancement. In two samples catalase activity became very weak, but catalase antigenicity was well preserved. The findings prove the diagnostic value of post mortem samples, even after extreme conditions of tissue conservation. The technique of immunocytochemical staining for the peroxisomal beta-oxidation enzymes on unmounted cryostat sections has not been reported previously. This method allows a quick diagnosis of biopsies from patients suspected of peroxisomal disorders.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0018-2214
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
57-62
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Immunocytochemical detection of peroxisomal beta-oxidation enzymes in cryostat and paraffin sections of human post mortem liver.
pubmed:affiliation
Department of Human Anatomy, Vrije Universiteit Brussel, Belgium.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't