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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1990-4-25
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pubmed:abstractText |
We have developed two monoclonal antibodies to human lipocortin-1 (103 and 105) as reagents for quantitating the protein in biological systems and neutralizing its activity. Lipo 105 is a high affinity antibody that is functional in ELISA and Western blot formats. The antibody recognizes a site between amino acids 30 and 55 in the lipocortin-1 sequence and can be used on native or denatured protein. Lipo 103 is an antibody that neutralizes the phospholipase A2 inhibitory activity of lipocortin-1 by blocking binding of the protein to phospholipid surfaces. The antibody is specific for native human lipocortin-1. Lipo 103 was recently shown to block lipocortin-1-dependent differentiation of a squamous carcinoma cell line, demonstrating its usefulness as a probe for function.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Annexins,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyanogen Bromide,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipases A,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipases A2,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
261
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
247-52
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1690147-Animals,
pubmed-meshheading:1690147-Annexins,
pubmed-meshheading:1690147-Antibodies, Monoclonal,
pubmed-meshheading:1690147-Antibody Specificity,
pubmed-meshheading:1690147-Blotting, Western,
pubmed-meshheading:1690147-Calcium-Binding Proteins,
pubmed-meshheading:1690147-Cyanogen Bromide,
pubmed-meshheading:1690147-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1690147-Epitopes,
pubmed-meshheading:1690147-Female,
pubmed-meshheading:1690147-Humans,
pubmed-meshheading:1690147-Indicators and Reagents,
pubmed-meshheading:1690147-Mice,
pubmed-meshheading:1690147-Mice, Inbred BALB C,
pubmed-meshheading:1690147-Peptide Fragments,
pubmed-meshheading:1690147-Peptide Mapping,
pubmed-meshheading:1690147-Phospholipases A,
pubmed-meshheading:1690147-Phospholipases A2,
pubmed-meshheading:1690147-Recombinant Proteins
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pubmed:year |
1990
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pubmed:articleTitle |
Monoclonal antibodies to lipocortin-1 as probes for biological function.
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pubmed:affiliation |
Biogen Inc., Cambridge, MA 02142.
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pubmed:publicationType |
Journal Article
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