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rdf:type |
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|
lifeskim:mentions |
umls-concept:C0003241,
umls-concept:C0003316,
umls-concept:C0003873,
umls-concept:C0019409,
umls-concept:C0024141,
umls-concept:C0030705,
umls-concept:C0033684,
umls-concept:C0035544,
umls-concept:C0035820,
umls-concept:C0205171,
umls-concept:C0444669,
umls-concept:C0521447,
umls-concept:C1167298,
umls-concept:C2347930
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|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1990-3-23
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|
pubmed:abstractText |
Antibodies to recombinant heterogeneous nuclear RNP core protein A1 were detected in sera from 27 of 58 patients with rheumatoid arthritis (RA) and from 7 of 31 patients with systemic lupus erythematosus, by immunoblotting and enzyme-linked immunosorbent assay. Protein A1 consists of 2 distinct domains: The N-terminal sequence is identical to a single-stranded DNA binding protein termed UP1, and the C-terminal domain shows a partial homology with keratin. All 7 A1-positive systemic lupus erythematosus sera reacted with UP1, whereas 9 of the 27 A1-positive RA sera did not. In RA, anti-A1 activity was significantly associated with antikeratin antibodies (AKA); these antibodies were present in 23 of 27 A1-positive sera and 10 of 31 A1-negative sera (P less than 0.01). Immunoabsorption with recombinant protein A1 resulted in a significant reduction of AKA titers in 6 of 10 RA sera tested, suggesting that AKA from RA patients may cross-react with the C-terminal portion of the heterogeneous nuclear RNP protein A1.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0004-3591
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
33
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
180-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1689580-Adolescent,
pubmed-meshheading:1689580-Adult,
pubmed-meshheading:1689580-Aged,
pubmed-meshheading:1689580-Arthritis, Rheumatoid,
pubmed-meshheading:1689580-Autoantibodies,
pubmed-meshheading:1689580-Autoantigens,
pubmed-meshheading:1689580-Cross Reactions,
pubmed-meshheading:1689580-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1689580-Epitopes,
pubmed-meshheading:1689580-Female,
pubmed-meshheading:1689580-Humans,
pubmed-meshheading:1689580-Immunoblotting,
pubmed-meshheading:1689580-Keratins,
pubmed-meshheading:1689580-Lupus Erythematosus, Systemic,
pubmed-meshheading:1689580-Male,
pubmed-meshheading:1689580-Middle Aged,
pubmed-meshheading:1689580-Nuclear Proteins,
pubmed-meshheading:1689580-Recombinant Proteins,
pubmed-meshheading:1689580-Viral Core Proteins,
pubmed-meshheading:1689580-snRNP Core Proteins
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|
pubmed:year |
1990
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pubmed:articleTitle |
Antibodies from patients with rheumatoid arthritis and systemic lupus erythematosus recognize different epitopes of a single heterogeneous nuclear RNP core protein. Possible role of cross-reacting antikeratin antibodies.
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|
pubmed:affiliation |
Istituto di Patologia Medica, IRCCS Policlinico S. Matteo, University of Pavia, Italy.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
