Source:http://linkedlifedata.com/resource/pubmed/id/16894355
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
2006-9-19
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pubmed:abstractText |
Although the antiangiogenic activity of type I interferons (IFN) is well known, the mechanism by which it occurs is unclear. In the present study, we have investigated effects of short-term and long-term IFN-alpha exposure on different types of endothelial cells (EC). Short-term IFN-alpha treatment resulted in a distinct reduction of apoptosis of serum and growth factor starved HUVEC and HDMEC. This was accompanied by a strong upregulation of the IFN inducible guanylate binding protein-1 (GBP-1) whereas no consistent regulation of several known antiapoptotic proteins was evident. Stable transfection of HUVEC with an expression vector for GBP-1 mimicked the protective effect of IFN-alpha, suggesting that GBP-1 may contribute to the inhibition of apoptosis. When IFN-alpha, together with serum and EC growth factors, was present continuously a decrease of population doublings by more than 40% was observed in both HDMEC and HCAEC. In addition, the cells displayed a senescent phenotype significantly earlier than control cells and showed an increased adherence for monocytes. Our findings suggest that the antiangiogenic effect of IFN-alpha is mediated by inducing EC senescence rather than EC apoptosis. Furthermore IFN-alpha released in chronic inflammatory conditions might contribute via its prosenescent activity to the pathogenesis of atherosclerosis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0023-6837
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
997-1007
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pubmed:meshHeading |
pubmed-meshheading:16894355-Apoptosis,
pubmed-meshheading:16894355-Atherosclerosis,
pubmed-meshheading:16894355-Capillaries,
pubmed-meshheading:16894355-Cell Aging,
pubmed-meshheading:16894355-Cells, Cultured,
pubmed-meshheading:16894355-Coronary Vessels,
pubmed-meshheading:16894355-Endothelial Cells,
pubmed-meshheading:16894355-GTP-Binding Proteins,
pubmed-meshheading:16894355-Humans,
pubmed-meshheading:16894355-Inhibitor of Differentiation Protein 1,
pubmed-meshheading:16894355-Interferon-alpha,
pubmed-meshheading:16894355-Neovascularization, Pathologic,
pubmed-meshheading:16894355-Umbilical Veins,
pubmed-meshheading:16894355-Up-Regulation
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pubmed:year |
2006
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pubmed:articleTitle |
Interferon-alpha prevents apoptosis of endothelial cells after short-term exposure but induces replicative senescence after continuous stimulation.
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pubmed:affiliation |
Institute of Clinical Pathology, Medical University of Vienna, Vienna, Austria.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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