Linked Life Data

16892178

Source:http://linkedlifedata.com/resource/pubmed/id/16892178

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2006-8-7
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/RefSeq/NM_000151, http://linkedlifedata.com/resource/pubmed/xref/RefSeq/NM_000224, http://linkedlifedata.com/resource/pubmed/xref/RefSeq/NM_000353, http://linkedlifedata.com/resource/pubmed/xref/RefSeq/NM_000371, http://linkedlifedata.com/resource/pubmed/xref/RefSeq/NM_000477, http://linkedlifedata.com/resource/pubmed/xref/RefSeq/NM_000780, http://linkedlifedata.com/resource/pubmed/xref/RefSeq/NM_001085, http://linkedlifedata.com/resource/pubmed/xref/RefSeq/NM_001134, http://linkedlifedata.com/resource/pubmed/xref/RefSeq/NM_002046, http://linkedlifedata.com/resource/pubmed/xref/RefSeq/NM_002273, http://linkedlifedata.com/resource/pubmed/xref/RefSeq/NM_002276, http://linkedlifedata.com/resource/pubmed/xref/RefSeq/NM_002701, http://linkedlifedata.com/resource/pubmed/xref/RefSeq/NM_005651, http://linkedlifedata.com/resource/pubmed/xref/RefSeq/NM_021784
pubmed:abstractText
Human embryonic stem cells (hESCs) have enormous potential as a source of cells for cell replacement therapies and as a model for early human development. In this study we examined the differentiating potential of hESCs into hepatocytes in two- and three-dimensional (2D and 3D) culture systems. Embryoid bodies (EBs) were inserted into a collagen scaffold 3D culture system or cultured on collagen-coated dishes and stimulated with exogenous growth factors to induce hepatic histogenesis. Immunofluorescence analysis revealed the expression of albumin (ALB) and cytokeratin-18 (CK-18). The differentiated cells in 2D and 3D culture system displayed several characteristics of hepatocytes, including expression of transthyretin, alpha-1-antitrypsin, cytokeratin 8, 18, 19, tryptophan-2,3-dioxygenase, tyrosine aminotransferase, glucose-6-phosphatase (G6P), cytochrome P450 subunits 7a1 and secretion of alpha-fetoprotein (AFP) and ALB and production of urea. In 3D culture, ALB and G6P were detected earlier and higher levels of urea and AFP were produced, when compared with 2D culture. Electron microscopy of differentiated hESCs showed hepatocyte-like ultrastructure, including glycogon granules, well-developed Golgi apparatuses, rough and smooth endoplasmic reticuli and intercellular canaliculi. The differentiation of hESCs into hepatocyte-like cells within 3D collagen scaffolds containing exogenous growth factors, gives rise to cells displaying morphological features, gene expression patterns and metabolic activities characteristic of hepatocytes and may provide a source of differentiated cells for treatment of liver diseases.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0214-6282
pubmed:author
pubmed:issnType
Print
pubmed:volume
50
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
645-52
pubmed:dateRevised
2007-8-28
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Differentiation of human embryonic stem cells into hepatocytes in 2D and 3D culture systems in vitro.
pubmed:affiliation
Department of Stem Cells, Royan Institute, Tehran, Iran. Baharvand50@yahoo.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't