Source:http://linkedlifedata.com/resource/pubmed/id/16892050
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9
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pubmed:dateCreated |
2006-8-21
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pubmed:databankReference | |
pubmed:abstractText |
Small-molecule inhibitors of kinesin-5 (refs. 1-3), a protein essential for eukaryotic cell division, represent alternatives to antimitotic agents that target tubulin. While tubulin is needed for multiple intracellular processes, the known functions of kinesin-5 are limited to dividing cells, making it likely that kinesin-5 inhibitors would have fewer side effects than do tubulin-targeting drugs. Kinesin-5 inhibitors, such as monastrol, act through poorly understood allosteric mechanisms, not competing with ATP binding. Moreover, the microscopic mechanism of full-length kinesin-5 motility is not known. Here we characterize the motile properties and allosteric inhibition of Eg5, a vertebrate kinesin-5, using a GFP fusion protein in single-molecule fluorescence assays. We find that Eg5 is a processive kinesin whose motility includes, in addition to ATP-dependent directional motion, a diffusive component not requiring ATP hydrolysis. Monastrol suppresses the directional processive motility of microtubule-bound Eg5. These data on Eg5's allosteric inhibition will impact these inhibitors' use as probes and development as chemotherapeutic agents.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antimitotic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Eg5 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Kinesin,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrimidines,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Thiones,
http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/monastrol
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1552-4450
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
480-5
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:16892050-Allosteric Regulation,
pubmed-meshheading:16892050-Animals,
pubmed-meshheading:16892050-Antimitotic Agents,
pubmed-meshheading:16892050-Green Fluorescent Proteins,
pubmed-meshheading:16892050-Kinesin,
pubmed-meshheading:16892050-Microscopy, Fluorescence,
pubmed-meshheading:16892050-Microtubules,
pubmed-meshheading:16892050-Mitotic Spindle Apparatus,
pubmed-meshheading:16892050-Pyrimidines,
pubmed-meshheading:16892050-Recombinant Fusion Proteins,
pubmed-meshheading:16892050-Thiones,
pubmed-meshheading:16892050-Xenopus Proteins,
pubmed-meshheading:16892050-Xenopus laevis
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pubmed:year |
2006
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pubmed:articleTitle |
Allosteric inhibition of kinesin-5 modulates its processive directional motility.
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pubmed:affiliation |
Laboratory of Chemistry and Cell Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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