Linked Life Data

16888712

Source:http://linkedlifedata.com/resource/pubmed/id/16888712

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
2006-9-6
pubmed:abstractText
Several primary amines are known to be endogenous substances, and they are to be detected at very low concentration level. Novel water-soluble azo dyes as precolumn derivatizing reagents were synthesized for the analysis of primary amines with strong visible-light absorption by CZE. A coupling reaction was used to introduce a sulfophenylazo group to salicylaldehyde or 1-hydroxy-2-naphthaldehyde to extend the conjugation and to improve the solubility in water of the derivatizing reagents, as well as the target compounds of the reaction products with the primary amines. Two azo dyes of 5-(4-sulfophenylazo)salicylaldehyde sodium salt (AZO1) and 4-(4-sulfophenylazo)-1-hydroxy-2-naphthaldehyde sodium salt (AZO2) were synthesized. Separation of eight kinds of n-alkylamines was performed by CZE after the derivatization with AZO1 or AZO2. The addition of Brij 35 to the running electrolyte and to the sample solutions was necessary to improve the solubility and the resolution among the analytes. Both reagents, AZO1 and AZO2, were compared with each other in terms of the reaction time of derivatization, the detection sensitivity and the total analysis time. The derivatization process using AZO2 was applied to the determination of four biogenic amines, histamine, tyramine, cadaverine and putrescine, and LODs at around micromolar level were achieved for the four amines.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0173-0835
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3460-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Synthesis of water-soluble phenylazosalicylaldehyde analogues and their application to capillary electrophoretic determination of primary amines.
pubmed:affiliation
The Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Validation Studies