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pubmed-article:1688806pubmed:abstractTextRNA accumulation in Chlamydomonas reinhardtii during the course of wall regeneration in protoplasts prepared by treatment of vegetative cells with autolysin was studied. In vitro translation of poly(A)+ RNA isolated from wall-regenerating cells indicated that cell wall removal induced the accumulation of several large RNA transcripts. Some of these induced RNAs were enriched following oligo(dG)-cellulose chromatography (referred to as cytosine-rich). The induced and cytosine-rich poly(A)+ RNAs potentially code for hydroxyproline-rich cell wall glycoproteins (HRGPs). Immunoprecipitation of in vitro translated products with an antiserum against a deglycosylated wall fraction (2BII) confirmed that these RNAs contained species involved in cell wall biosynthesis.lld:pubmed
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pubmed-article:1688806pubmed:articleTitleInduction of cytosine-rich poly(A)+ RNAs in Chlamydomonas reinhardtii by cell wall removal.lld:pubmed
pubmed-article:1688806pubmed:affiliationDepartment of Biological Sciences, University of California, Santa Barbara 93106.lld:pubmed
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