rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1990-3-15
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pubmed:abstractText |
RNA accumulation in Chlamydomonas reinhardtii during the course of wall regeneration in protoplasts prepared by treatment of vegetative cells with autolysin was studied. In vitro translation of poly(A)+ RNA isolated from wall-regenerating cells indicated that cell wall removal induced the accumulation of several large RNA transcripts. Some of these induced RNAs were enriched following oligo(dG)-cellulose chromatography (referred to as cytosine-rich). The induced and cytosine-rich poly(A)+ RNAs potentially code for hydroxyproline-rich cell wall glycoproteins (HRGPs). Immunoprecipitation of in vitro translated products with an antiserum against a deglycosylated wall fraction (2BII) confirmed that these RNAs contained species involved in cell wall biosynthesis.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Mar
|
pubmed:issn |
0014-4827
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
187
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
54-8
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:1688806-Blotting, Northern,
pubmed-meshheading:1688806-Cell Wall,
pubmed-meshheading:1688806-Chlamydomonas,
pubmed-meshheading:1688806-Cycloheximide,
pubmed-meshheading:1688806-Cytosine,
pubmed-meshheading:1688806-Kinetics,
pubmed-meshheading:1688806-Poly A,
pubmed-meshheading:1688806-Poly G,
pubmed-meshheading:1688806-Protein Biosynthesis,
pubmed-meshheading:1688806-Protoplasts,
pubmed-meshheading:1688806-RNA,
pubmed-meshheading:1688806-RNA, Messenger,
pubmed-meshheading:1688806-Tunicamycin
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pubmed:year |
1990
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pubmed:articleTitle |
Induction of cytosine-rich poly(A)+ RNAs in Chlamydomonas reinhardtii by cell wall removal.
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pubmed:affiliation |
Department of Biological Sciences, University of California, Santa Barbara 93106.
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pubmed:publicationType |
Journal Article
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