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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 16
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pubmed:dateCreated |
2006-8-4
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pubmed:abstractText |
The molecular mechanisms underlying muscle atrophy during spaceflight are not well understood. We have analyzed the effects of a 10-day spaceflight on Caenorhabditis elegans muscle development. DNA microarray, real-time quantitative PCR, and quantitative western blot analyses revealed that the amount of MHC in both body-wall and pharyngeal muscle decrease in response to spaceflight. Decreased transcription of the body-wall myogenic transcription factor HLH-1 (CeMyoD) and of the three pharyngeal myogenic transcription factors, PEB-1, CEH-22 and PHA-4 were also observed. Upon return to Earth animals displayed reduced rates of movement, indicating a functional defect. These results demonstrate that C. elegans muscle development is altered in response to spaceflight. This altered development occurs at the level of gene transcription and was observed in the presence of innervation, not simply in isolated cells. This important finding coupled with past observations of decreased levels of the same myogenic transcription factions in vertebrates after spaceflight raises the possibility that altered muscle development is a contributing factor to spaceflight-induced muscle atrophy in vertebrates.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CEH-22 protein, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HLH-1 protein, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Myogenic Regulatory Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Myosin Heavy Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Pha-4 protein, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tropomyosin,
http://linkedlifedata.com/resource/pubmed/chemical/peb-1 protein, C elegans
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-0949
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
209
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3209-18
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16888068-Animals,
pubmed-meshheading:16888068-Caenorhabditis elegans,
pubmed-meshheading:16888068-Caenorhabditis elegans Proteins,
pubmed-meshheading:16888068-DNA-Binding Proteins,
pubmed-meshheading:16888068-Gene Expression Profiling,
pubmed-meshheading:16888068-Gene Expression Regulation, Developmental,
pubmed-meshheading:16888068-Homeodomain Proteins,
pubmed-meshheading:16888068-Muscle Development,
pubmed-meshheading:16888068-Muscles,
pubmed-meshheading:16888068-Myogenic Regulatory Factors,
pubmed-meshheading:16888068-Myosin Heavy Chains,
pubmed-meshheading:16888068-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:16888068-Polymerase Chain Reaction,
pubmed-meshheading:16888068-RNA, Messenger,
pubmed-meshheading:16888068-Space Flight,
pubmed-meshheading:16888068-Trans-Activators,
pubmed-meshheading:16888068-Transcription Factors,
pubmed-meshheading:16888068-Tropomyosin
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pubmed:year |
2006
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pubmed:articleTitle |
Decreased expression of myogenic transcription factors and myosin heavy chains in Caenorhabditis elegans muscles developed during spaceflight.
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pubmed:affiliation |
Japan Aerospace Exploration Agency, 2-1-1, Sengen, Tsukuba, Ibaraki 305-8505, Japan. higashibata.akira@jaxa.jp
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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