Linked Life Data

16887185

Source:http://linkedlifedata.com/resource/pubmed/id/16887185

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-12-18
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY177629, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY242386, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY267211, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY267212, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY267213, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY293927, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY293928, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY293929, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY303809, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY303810, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY303811, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY303812, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY303813, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY452069, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ291157, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ291158
pubmed:abstractText
Virus infection of mammalian cells activates an innate antiviral immune response characterized by production of interferon (IFN) and the subsequent transcriptional upregulation of IFN-stimulated genes (ISGs) by the JAK-STAT signaling pathway. Here, we report that a fish cell line, crucian carp (Carassius auratus L.) blastulae embryonic (CAB) cells, can produce IFN activity and then form an antiviral state after infection with UV-inactivated grass carp hemorrhagic virus (GCHV), a double-stranded (ds) RNA virus. From UV-inactivated GCHV-infected CAB cells, 15 pivotal genes were cloned and sequenced, and all of them were shown to be involved in IFN antiviral innate immune response. These IFN system genes include the dsRNA signal sensing factor TLR3, IFN, IFN signal transduction factor STAT1, IFN regulatory factor IRF7, putative IFN antiviral effectors Mx1, Mx2, PKR-like, Viperin, IFI56, and other IFN stimulated genes (ISGs) IFI58, ISG15-1, ISG15-2, USP18, Gig1 and Gig2. The identified fish IFN system genes were highly induced by active GCHV, UV-inactivated GCHV, CAB IFN or poly(I).poly(C), and showed similar expression patterns to mammals. The data indicate that an IFN antiviral innate immune response similar to that in mammals exists in the UV-inactivated GCHV-infected CAB cells, and the IFN response contributes to the formation of an antiviral state probably through JAK-STAT signaling pathway. This study provides strong evidence for existence of IFN antiviral innate immune response in fish, and will assist in elucidating the origin and evolution of vertebrate IFN system.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0145-305X
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
232-43
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
The innate immune response to grass carp hemorrhagic virus (GCHV) in cultured Carassius auratus blastulae (CAB) cells.
pubmed:affiliation
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Wuhan 430072, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't