Linked Life Data

16887100

Source:http://linkedlifedata.com/resource/pubmed/id/16887100

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2006-8-14
pubmed:abstractText
The adenine nucleotide translocase (ANT), besides transferring ATP from the mitochondrial matrix to the rest of the cell, has also been proposed to be involved in mitochondrial permeability transition (MPT), and accordingly in mitochondrial Ca2+ homeostasis. In order to assess the role of ANT in Ca2+ signal transmission from the endoplasmic reticulum (ER) to mitochondria, we overexpressed the various ANT isoforms and measured the matrix [Ca2+] ([Ca2+]m) increases evoked by stimulation with IP3-dependent agonists. ANT overexpression reduced the amplitude of the [Ca2+]m peak following Ca2+ release, an effect that was markedly greater for ANT-1 and ANT-3 isoforms than for ANT-2. Three further observations might explain these findings. First, the effect was partially reversed by treating the cells with cyclosporine A, suggesting the involvement of MPT. Second, the effect was paralleled by alterations of the 3D structure of the mitochondria. Finally, ANT-1 and ANT-3 overexpression also caused a reduction of ER Ca2+ loading that caused a marginal decrease in the cytosolic Ca2+ responses. Overall, these data provide evidence for the involvement of ANT-1 and ANT-3 in the induction of MPT and indicate the relevance of this phenomenon in ER-mitochondria Ca2+ transfer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
348
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
393-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Overexpression of adenine nucleotide translocase reduces Ca2+ signal transmission between the ER and mitochondria.
pubmed:affiliation
Department of Experimental and Diagnostic Medicine, Section of General Pathology, Interdisciplinary Center for the Study of Inflammation (ICSI), Emilia Romagna Laboratory for Genomics and Biotechnology (ER-Gentech), University of Ferrara, Ferrara, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't