rdf:type |
|
lifeskim:mentions |
umls-concept:C0015733,
umls-concept:C0016710,
umls-concept:C0025663,
umls-concept:C0032520,
umls-concept:C0035950,
umls-concept:C0205147,
umls-concept:C0220825,
umls-concept:C0332324,
umls-concept:C0392355,
umls-concept:C0392762,
umls-concept:C1511790,
umls-concept:C1551666
|
pubmed:issue |
8
|
pubmed:dateCreated |
2006-8-3
|
pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ364808,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ364809,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ364810,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ364811,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ364812,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ364813,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ364814,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ364815,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ364816,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ364817,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ364818,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ364819,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ364820,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ364821,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/DQ364822
|
pubmed:abstractText |
A quantitative TaqMan minor-groove binder real-time PCR assay was developed for the sensitive detection of a ruminant-specific genetic marker in fecal members of the phylum Bacteroidetes. The qualitative and quantitative detection limits determined were 6 and 20 marker copies per PCR, respectively. Tested ruminant feces contained an average of 4.1 x 10(9) marker equivalents per g, allowing the detection of 1.7 ng of feces per filter in fecal suspensions. The marker was detected in water samples from a karstic catchment area at levels matching a gradient from negligible to considerable ruminant fecal influence (from not detectable to 10(5) marker equivalents per liter).
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pubmed:grant |
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-10543789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-10606668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-10742246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-11010920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-11055900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-11097934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-11320111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-11375195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-11714526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-12521151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-12531273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-12620869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-12636264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-12957899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-15325762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-15658992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-15933020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-16011762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-16204514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-3884562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-3919638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-6706881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-7538270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-8919802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-9185578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16885315-9758854
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Aug
|
pubmed:issn |
0099-2240
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
72
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
5610-4
|
pubmed:dateRevised |
2011-4-6
|
pubmed:meshHeading |
pubmed-meshheading:16885315-Animals,
pubmed-meshheading:16885315-Bacteroidetes,
pubmed-meshheading:16885315-Feces,
pubmed-meshheading:16885315-Fresh Water,
pubmed-meshheading:16885315-Genetic Markers,
pubmed-meshheading:16885315-Molecular Sequence Data,
pubmed-meshheading:16885315-Polymerase Chain Reaction,
pubmed-meshheading:16885315-Ruminants,
pubmed-meshheading:16885315-Sensitivity and Specificity,
pubmed-meshheading:16885315-Sequence Analysis, DNA,
pubmed-meshheading:16885315-Water Pollution,
pubmed-meshheading:16885315-Water Supply
|
pubmed:year |
2006
|
pubmed:articleTitle |
Quantitative PCR method for sensitive detection of ruminant fecal pollution in freshwater and evaluation of this method in alpine karstic regions.
|
pubmed:affiliation |
Institute for Chemical Engineering, Gene Technology Group, Vienna University of Technology, Getreidemarkt 9-166/5, A-1060 Vienna, Austria.
|
pubmed:publicationType |
Journal Article,
Evaluation Studies
|