Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-8-15
pubmed:databankReference
pubmed:abstractText
Three single-chain variable fragment (scFv) antibodies recognizing the nuclear inclusion a (NIa) and capsid proteins of potato virus Y were obtained from two mouse derived hybridoma clones secreting, respectively, an anti-NIa (22-1) and an anti-coat protein (136-13) monoclonal antibodies. The first monoclonal antibody was able to inhibit in vitro the PVY polyprotein cleavage by blocking the NIa protease activity. The amplified scFv cDNAs were first inserted into the TOPO vector and then sequenced. Several recombinant E. coli clones carrying the accurate scFv sequences were selected and the corresponding cDNAs were subcloned in pHEN phagemid and transferred in E. coli strain. The expressed scFv fragments showed an antibody activity that recognized the viral target proteins in infected tissues. Their activity was comparable to the parental monoclonal antibodies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0166-0934
pubmed:author
pubmed:issnType
Print
pubmed:volume
137
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-6
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:16884787-Amino Acid Sequence, pubmed-meshheading:16884787-Antibodies, Monoclonal, pubmed-meshheading:16884787-Antibodies, Viral, pubmed-meshheading:16884787-Capsid Proteins, pubmed-meshheading:16884787-Cloning, Molecular, pubmed-meshheading:16884787-DNA, Complementary, pubmed-meshheading:16884787-Endopeptidases, pubmed-meshheading:16884787-Escherichia coli, pubmed-meshheading:16884787-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:16884787-Hybridomas, pubmed-meshheading:16884787-Immunoglobulin Variable Region, pubmed-meshheading:16884787-Molecular Sequence Data, pubmed-meshheading:16884787-Potyvirus, pubmed-meshheading:16884787-Recombinant Proteins, pubmed-meshheading:16884787-Sequence Analysis, DNA, pubmed-meshheading:16884787-Solanum tuberosum, pubmed-meshheading:16884787-Viral Proteins
pubmed:year
2006
pubmed:articleTitle
Cloning and expression of functional single-chain Fv antibodies directed against NIa and coat proteins of potato virus Y.
pubmed:affiliation
Centre de Biotechnologie de Sfax, BP K, 3018 Sfax, Tunisia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't