Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
2006-8-2
pubmed:abstractText
Initiation of DNA replication in Eschericia coli requires the ATP-bound form of the DnaA protein. The conversion of DnaA-ATP to DnaA-ADP is facilitated by a complex of DnaA, Hda (homologous to DnaA), and DNA-loaded beta-clamp proteins in a process termed RIDA (regulatory inactivation of DnaA). Hda-deficient cells initiate replication at each origin mainly once per cell cycle, and the rare reinitiation events never coincide with the end of the origin sequestration period. Therefore, RIDA is not the predominant mechanism to prevent immediate reinitiation from oriC. The cellular level of Hda correlated directly with dnaA gene expression such that Hda deficiency led to reduced dnaA gene expression, and overproduction of Hda led to DnaA overproduction. Hda-deficient cells were very sensitive to variations in the cellular level of DnaA, and DnaA overproduction led to uncontrolled initiation of replication from oriC, causing severe growth retardation or cell death. Based on these observations, we propose that both RIDA and dnaA gene autoregulation are required as homeostatic mechanisms to ensure that initiation of replication occurs at the same time relative to cell mass in each cell cycle.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-10545126, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-10660546, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-10801458, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-10811905, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-11080169, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-11250912, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-11263490, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-11483528, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-11690638, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-11839737, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-12067334, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-12682301, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-12730188, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-14507385, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-14643657, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-14756777, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-149110, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-14978287, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-15123823, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-15189445, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-15611053, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-15708977, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-16041320, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-16077105, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-16359333, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-16482221, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-1697508, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-1925010, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-1925015, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-2153124, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-2540407, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-2541928, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-2835364, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-2981626, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-3036372, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-3057497, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-3527695, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-4866337, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-4876941, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-6271456, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-6296774, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-7891562, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-8011018, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-8071330, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-8168484, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-8830270, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-8918477, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-9140960, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-9278503, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-9478970, http://linkedlifedata.com/resource/pubmed/commentcorrection/16882985-9674428
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0890-9369
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2121-34
pubmed:dateRevised
2010-8-12
pubmed:meshHeading
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