|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
16
|
|
pubmed:dateCreated |
2006-8-2
|
|
pubmed:abstractText |
Consumption of fresh apples can cause allergy in susceptible individuals. A competitive enzyme-linked immunosorbent assay (ELISA) has been developed to determine Mal d 1 levels in apple pulp using a monoclonal antibody (BIP-1). The ELISA was able to rank ten cultivars according to their Mal d 1 content (between 3.8 and 72.5 mug/g pulp). For the first time, it has been demonstrated that growing conditions and postharvest storage, using three different treatments over a 5 month period in 2 consecutive years, increase Mal d 1 expression at a translational and transcriptional level (3.5- and 8.5-fold under controlled atmosphere storage). Expression of three major Mal d 1 isoforms was observed by real-time polymerase chain reaction over the 5 month storage period, and Mal d 1.02 was the most highly expressed isoform. In conclusion, Mal d 1 gene expression was significantly increased during modified atmosphere storage. Individuals suffering from birch pollen-apple allergy syndrome might experience fewer problems consuming freshly picked apples.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0021-8561
|
|
pubmed:author |
pubmed-author:BrowneTomT,
pubmed-author:DeyRickmerR,
pubmed-author:FoxallRobertR,
pubmed-author:Hoffmann-SommergruberKarinK,
pubmed-author:LaimerMargitM,
pubmed-author:MarzbanGorjiG,
pubmed-author:MillsE N ClareEN,
pubmed-author:SanchoAna IAI,
pubmed-author:WaldronKeith WKW,
pubmed-author:ZuidmeerLaurianL,
pubmed-author:van ReeRonaldR
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
9
|
|
pubmed:volume |
54
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
5917-23
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:16881695-Allergens,
pubmed-meshheading:16881695-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:16881695-Food Preservation,
pubmed-meshheading:16881695-Fruit,
pubmed-meshheading:16881695-Gene Expression,
pubmed-meshheading:16881695-Malus,
pubmed-meshheading:16881695-Plant Proteins,
pubmed-meshheading:16881695-RNA, Messenger,
pubmed-meshheading:16881695-RNA, Plant,
pubmed-meshheading:16881695-Reproducibility of Results,
pubmed-meshheading:16881695-Time Factors
|
|
pubmed:year |
2006
|
|
pubmed:articleTitle |
Effect of postharvest storage on the expression of the apple allergen Mal d 1.
|
|
pubmed:affiliation |
Institute of Food Research, Norwich Research Park, Colney, UK. ana.sancho@bbsrc.ac.uk
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
