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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
32
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pubmed:dateCreated |
2006-8-9
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pubmed:abstractText |
Genes involved in a viral resistance response in common bean (Phaseolus vulgaris cv. Othello) were identified by inoculating a geminivirus reporter (Bean dwarf mosaic virus expressing the green fluorescent protein), extracting RNA from tissue undergoing the defense response, and amplifying sequences with degenerate R gene primers. One such gene (a TIR-NBS-LRR gene, RT4-4) was selected for functional analysis in which transgenic Nicotiana benthamiana were generated and screened for resistance to a range of viruses. This analysis revealed that RT4-4 did not confer resistance to the reporter geminivirus; however, it did activate a resistance-related response (systemic necrosis) to seven strains of Cucumber mosaic virus (CMV) from pepper or tomato, but not to a CMV strain from common bean. Of these eight CMV strains, only the strain from common bean systemically infected common bean cv. Othello. Additional evidence that RT4-4 is a CMV R gene came from the detection of resistance response markers in CMV-challenged leaves of RT4-4 transgenic plants, and the identification of the CMV 2a gene product as the elicitor of the necrosis response. These findings indicate that RT4-4 functions across two plant families and is up-regulated in a non-virus-specific manner. This experimental approach holds promise for providing insights into the mechanisms by which plants activate resistance responses against pathogens.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16880399-10330465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16880399-10570214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16880399-10652152,
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
103
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11856-61
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:16880399-Cloning, Molecular,
pubmed-meshheading:16880399-Cucumovirus,
pubmed-meshheading:16880399-Fabaceae,
pubmed-meshheading:16880399-Gene Expression Regulation, Plant,
pubmed-meshheading:16880399-Genes, Plant,
pubmed-meshheading:16880399-Genes, Viral,
pubmed-meshheading:16880399-Models, Genetic,
pubmed-meshheading:16880399-Mutagenesis, Site-Directed,
pubmed-meshheading:16880399-Necrosis,
pubmed-meshheading:16880399-Plant Diseases,
pubmed-meshheading:16880399-Plants, Genetically Modified,
pubmed-meshheading:16880399-RNA,
pubmed-meshheading:16880399-Transgenes,
pubmed-meshheading:16880399-Up-Regulation
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pubmed:year |
2006
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pubmed:articleTitle |
A viral resistance gene from common bean functions across plant families and is up-regulated in a non-virus-specific manner.
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pubmed:affiliation |
Department of Plant Pathology and Section of Plant Biology, University of California, Davis, CA 95616, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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