Source:http://linkedlifedata.com/resource/pubmed/id/16879654
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2006-8-1
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pubmed:abstractText |
An L-galactonate dehydratase and the corresponding gene were identified from the mould Hypocrea jecorina (Trichoderma reesei). This novel enzyme converts L-galactonate to L-threo-3-deoxy-hexulosonate (2-keto-3-deoxy-L-galactonate). The enzyme is part of the fungal pathway for D-galacturonic acid catabolism, a pathway which is only partly known. It is the second enzyme of this pathway after the D-galacturonic acid reductase. L-galactonate dehydratase activity is present in H. jecorina cells grown on D-galacturonic acid but absent when other carbon sources are used for growth. A deletion of the L-galactonate dehydratase gene in H. jecorina results in a strain with no growth on D-galacturonic acid. The active enzyme was produced in the heterologous host Saccharomyces cerevisiae and characterized. It exhibited activity with L-galactonate and D-arabonate where the hydroxyl group of the C2 is in L- and the hydroxyl group of the C3 is in D-configuration in the Fischer projection. However, it did not exhibit activity with D-galactonate, D-gluconate, L-gulonate or D-xylonate where the hydroxyl groups of the C2 and C3 are in different configuration.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glyceraldehyde,
http://linkedlifedata.com/resource/pubmed/chemical/Glycerol,
http://linkedlifedata.com/resource/pubmed/chemical/Hexuronic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Hydro-Lyases,
http://linkedlifedata.com/resource/pubmed/chemical/Pyruvic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/galactonate dehydratase,
http://linkedlifedata.com/resource/pubmed/chemical/galacturonic acid
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0950-382X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1060-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16879654-Blotting, Northern,
pubmed-meshheading:16879654-Cloning, Molecular,
pubmed-meshheading:16879654-Fungal Proteins,
pubmed-meshheading:16879654-Gene Deletion,
pubmed-meshheading:16879654-Glyceraldehyde,
pubmed-meshheading:16879654-Glycerol,
pubmed-meshheading:16879654-Hexuronic Acids,
pubmed-meshheading:16879654-Hydro-Lyases,
pubmed-meshheading:16879654-Hypocrea,
pubmed-meshheading:16879654-Magnetic Resonance Spectroscopy,
pubmed-meshheading:16879654-Pyruvic Acid
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pubmed:year |
2006
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pubmed:articleTitle |
L-galactonate dehydratase is part of the fungal path for D-galacturonic acid catabolism.
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pubmed:affiliation |
VTT Biotechnology, Espoo, Finland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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