Linked Life Data

16876394

Source:http://linkedlifedata.com/resource/pubmed/id/16876394

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2006-10-18
pubmed:abstractText
Articular cartilage functions in dissipating forces applied across joints. It comprises an extracellular matrix containing primarily collagens, proteoglycans and water to maintain its functional properties, and is interspersed with chondrocytes. The chondrocyte cytoskeleton comprises actin microfilaments, tubulin microtubules and vimentin intermediate filaments. Previous studies have determined the contribution of actin and tubulin in regulating the synthesis of the extracellular matrix components aggrecan and type II collagen. The contribution of vimentin to extracellular matrix biosynthesis in any cell type has not previously been addressed. Therefore the aim of this study was to assess the role of vimentin in cartilage chondrocyte metabolism. Vimentin intermediate filaments were disrupted in high-density monolayer articular chondrocyte cultures using acrylamide for 7 days. De novo protein and collagen synthesis were measured by adding [3H]-proline, and sulphated glycosaminoglycan (sGAG) synthesis measured by adding [35S]-sulphate to cultures. Vimentin disruption resulted in decreased collagen synthesis, whilst sGAG synthesis was unaffected. In addition, there was a significant reduction in type II collagen and aggrecan gene transcription suggesting that the effects observed occur at both the transcriptional and translational levels. A 3-day cold chase demonstrated a significant inhibition of collagen and sGAG degradation; the reduction in collagen degradation was corroborated by the observed reduction in both pro-MMP 2 expression and activation. We have demonstrated that an intact vimentin intermediate filament network contributes to the maintenance of the chondrocyte phenotype and thus an imbalance favouring filament disassembly can disturb the integrity of the articular cartilage, and may ultimately lead to the development of pathologies such as osteoarthritis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0945-053X
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
398-408
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Disassembly of the vimentin cytoskeleton disrupts articular cartilage chondrocyte homeostasis.
pubmed:affiliation
Connective Tissue Biology Laboratories, Biomedical Sciences Building, School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3US, Wales, UK. BLAIN@cardiff.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural