Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1992-4-29
pubmed:databankReference
pubmed:abstractText
The 4.4 kb SphI DNA fragment (GSH1) that complements the gamma-glutamylcysteine synthetase-deficient mutation (gsh1) of Saccharomyces cerevisiae YH1 was cloned into vector plasmid YEp24. Gene disruption of the cloned fragment confirmed that this segment was the same gene as gsh1. Mutant strain YH1 with this plasmid not only restored gamma-glutamylcysteine synthetase (GSH-I) activity but the glutathione content and the growth rate. DNA sequence analysis of the SphI fragment showed that the GSH1 structural gene contained 2034 bp and predicted a polypeptide of 678 amino acids. The deduced amino acid sequence had about a 45% homology to that of rat kidney GSH-I, but a very low homology (about 26%) to that of Escherichia coli GSH-I. Northern analysis showed that GSH1 had been transcribed into an approximately 2.7 kb mRNA fragment. Southern analysis showed that GSH1 mapped at chromosome X.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0749-503X
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:geneSymbol
GSH1, LEU2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
953-61
pubmed:dateRevised
2006-5-1
pubmed:meshHeading
pubmed-meshheading:1687097-Amino Acid Sequence, pubmed-meshheading:1687097-Animals, pubmed-meshheading:1687097-Base Sequence, pubmed-meshheading:1687097-Blotting, Northern, pubmed-meshheading:1687097-Blotting, Southern, pubmed-meshheading:1687097-Chromosome Mapping, pubmed-meshheading:1687097-Cloning, Molecular, pubmed-meshheading:1687097-DNA, Fungal, pubmed-meshheading:1687097-Genetic Vectors, pubmed-meshheading:1687097-Glutamate-Cysteine Ligase, pubmed-meshheading:1687097-Kidney, pubmed-meshheading:1687097-Molecular Sequence Data, pubmed-meshheading:1687097-Mutation, pubmed-meshheading:1687097-Plasmids, pubmed-meshheading:1687097-Rats, pubmed-meshheading:1687097-Repetitive Sequences, Nucleic Acid, pubmed-meshheading:1687097-Restriction Mapping, pubmed-meshheading:1687097-Saccharomyces cerevisiae, pubmed-meshheading:1687097-TATA Box, pubmed-meshheading:1687097-Transcription, Genetic
pubmed:year
1991
pubmed:articleTitle
Molecular cloning of the gamma-glutamylcysteine synthetase gene of Saccharomyces cerevisiae.
pubmed:affiliation
Central Research Laboratories, Asahi Breweries Ltd., Tokyo, Japan.
pubmed:publicationType
Journal Article