Linked Life Data

1686999

Source:http://linkedlifedata.com/resource/pubmed/id/1686999

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1992-4-28
pubmed:abstractText
High molecular (designated Sv, greater than 3K) and low molecular (designated Sv, less than 3K) fractions were prepared by filtration of porcine vitreous extracts through a membrane filter with a molecular weight cut-off of 3,000 daltons. Both fractions inhibited mitogen-stimulated proliferation of lymphocytes from rat spleen, as determined by [3H]-thymidine incorporation. The inhibitory activity of Sv, greater than 3K was markedly enhanced by acid treatment and abolished almost completely by incubation with anti-transforming growth factor beta (TGF-beta) antibody. The major immunosuppressive factor in Sv, greater than 3K was therefore concluded to be TGF-beta. On the other hand, the inhibitory activity of Sv, less than 3K was not affected by acid treatment or by incubation with anti-TGF-beta antibody but was decreased by proteolytic treatment. From these results the immunosuppressive factor in Sv, less than 3K was attributed to a low molecular substance which is probably a peptide structurally unrelated to TGF-beta. The inhibitory effects of both Sv, greater than 3K and Sv, less than 3K were reversible and therefore not cytotoxic. Both factors seemed to suppress lymphoproliferation without affecting the expression of Interleukin-2 receptor, CD4 and CD8 on the cell surface of cultured lymphocytes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0271-3683
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1141-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Immunosuppressive factors in porcine vitreous body.
pubmed:affiliation
Kresge Eye Institute, Department of Ophthalmology, Wayne State University School of Medicine, Detroit, MI 48201.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.