Source:http://linkedlifedata.com/resource/pubmed/id/16867353
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6-8
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| pubmed:dateCreated |
2006-7-26
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| pubmed:abstractText |
A method is described for the simultaneous analysis of alpha-tocopherol, beta-carotene, and retinol in n-hexane extracts of serum. The technique uses normal-phase HPLC in a silica gel column with n-hexane-2-propanol (97:3, v/v) as the mobile phase. The compounds are eluted from the column in the order alpha-tocopherol, beta-carotene, tocol (internal standard) and retinol. The compounds are quantified by use of the following detectors connected in series: retinol and tocol by a spectrophotometer, beta-carotene by a filter photometer, and alpha-tocopherol and tocol by a spectrophotofluorimeter. The time taken for separation is less than 6 min but a further 14 min is allowed for elution of the more polar compounds before the next run. With an automatic injector, the method is suitable for the analysis of a large number of samples because of the relatively short time for analysis and the small volume (0.2 ml) of serum required. The method has been used in a study of the relationships between serum levels of the fat-soluble vitamins and subsequent cancer in 2332 subjects.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:issn |
0731-7085
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
6
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
853-7
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| pubmed:year |
1988
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| pubmed:articleTitle |
HPLC method for the simultaneous determination of beta-carotene, retinol and alpha-tocopherol in serum.
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| pubmed:affiliation |
Department of Biomedical Sciences, University of Tampere, P.O. Box 607, SF-33101 Tampere, Finland.
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| pubmed:publicationType |
Journal Article
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