Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2006-8-28
pubmed:abstractText
Dopamine (DA) neurotransmission has been implicated in several neurological and psychiatric disorders. The dopamine transporter (DAT) is highly expressed in dopaminergic neurons of the ventral mesencephalon and regulates neurotransmission by transporting DA back into the presynaptic terminals. To mediate restricted DNA recombination events into DA neurons using the Cre/loxP technology, we have generated a knockin mouse expressing Cre recombinase under the transcriptional control of the endogenous DAT promoter. To minimize interference with DAT function by preservation of both DAT alleles, Cre recombinase expression was driven from the 3' untranslated region (3'UTR) of the endogenous DAT gene by means of an internal ribosomal entry sequence. Crossing this murine line with a LacZ reporter showed colocalization of DAT immunocytochemistry and beta-galactosidase staining in all regions analyzed. This knockin mouse can be used for generating tissue specific knockouts in mice carrying genes flanked by loxP sites, and will facilitate the analysis of gene function in dopaminergic neurons.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1526-954X
pubmed:author
pubmed:copyrightInfo
Copyright 2006 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
383-90
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16865686-3' Untranslated Regions, pubmed-meshheading:16865686-Animals, pubmed-meshheading:16865686-Brain, pubmed-meshheading:16865686-Crosses, Genetic, pubmed-meshheading:16865686-Dopamine Plasma Membrane Transport Proteins, pubmed-meshheading:16865686-Female, pubmed-meshheading:16865686-Genes, Reporter, pubmed-meshheading:16865686-Heterozygote, pubmed-meshheading:16865686-Homozygote, pubmed-meshheading:16865686-Integrases, pubmed-meshheading:16865686-Lac Operon, pubmed-meshheading:16865686-Male, pubmed-meshheading:16865686-Mice, pubmed-meshheading:16865686-Mice, Inbred C57BL, pubmed-meshheading:16865686-Mice, Transgenic, pubmed-meshheading:16865686-Mutation, pubmed-meshheading:16865686-Neurons, pubmed-meshheading:16865686-Pregnancy, pubmed-meshheading:16865686-RNA, Messenger, pubmed-meshheading:16865686-Recombination, Genetic, pubmed-meshheading:16865686-Substantia Nigra, pubmed-meshheading:16865686-Ventral Tegmental Area, pubmed-meshheading:16865686-beta-Galactosidase
pubmed:year
2006
pubmed:articleTitle
Characterization of a mouse strain expressing Cre recombinase from the 3' untranslated region of the dopamine transporter locus.
pubmed:affiliation
Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA. cbackman@mail.nih.gov
pubmed:publicationType
Journal Article, Comparative Study, Research Support, N.I.H., Intramural