Linked Life Data

16865673

Source:http://linkedlifedata.com/resource/pubmed/id/16865673

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2006-12-4
pubmed:abstractText
Human Rex-1 (hRex-1) (also referred to as zinc-finger protein-42, Zfp42) encodes a zinc finger protein expression of which is believed to be characteristic of pluripotent stem cells. We have applied bioinformatics to classify the relationship of human, rat, and mouse REX1 proteins in the C2H2 family of zinc finger proteins and demonstrate that REX1 is a member of the YY1 sub-family of transcription factors, which includes the Drosophila pleiohomeotic (Pho) protein. We have generated a molecular model of the human REX1 zinc finger domains based on the crystal structure of the YY1 transcription factor. To date, expression of hRex-1 and its extensively studied mouse homolog mRex-1, has been reported only in embryonic and adult stem cells and in differentiated spermatocytes. In this study, reverse transcription-PCR and Western analysis were employed to assay for hRex-1 expression in cultured normal human epithelial cells and human carcinoma cell lines. Expression of hRex-1 mRNA was detected in normal human epidermal keratinocytes, normal prostate epithelial cells (PrEC), bronchial, and small airway lung epithelial cells. Other stem cell markers, such as Oct 4, DAB2, and cMyc were also detected in normal human epidermal keratinocyte cultures. Expression of hRex-1 was also detected in some human tumor cell lines including MDA-MB-468 mammary carcinoma, SCC-15 head and neck squamous cell carcinoma, and N-TERA2 human teratocarcinoma cells. Western analyses confirmed expression of the human REX1 (ZFP42) protein in MDA-MB-468 cells and normal human keratinocytes. This research has identified model human cell culture systems, in addition to embryonic stem (ES) cells, in which Rex-1 is expressed, and this should enable the characterization of REX1 functions in normal adult epithelial cells and tumorigenic stem cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0899-1987
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2006 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
887-900
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:16865673-Amino Acid Sequence, pubmed-meshheading:16865673-Animals, pubmed-meshheading:16865673-Biological Markers, pubmed-meshheading:16865673-Blotting, Western, pubmed-meshheading:16865673-Carcinoma, pubmed-meshheading:16865673-Carrier Proteins, pubmed-meshheading:16865673-Epithelial Cells, pubmed-meshheading:16865673-Humans, pubmed-meshheading:16865673-Kruppel-Like Transcription Factors, pubmed-meshheading:16865673-Mice, pubmed-meshheading:16865673-Models, Biological, pubmed-meshheading:16865673-Molecular Sequence Data, pubmed-meshheading:16865673-Nuclear Proteins, pubmed-meshheading:16865673-Protein Conformation, pubmed-meshheading:16865673-RNA, Messenger, pubmed-meshheading:16865673-Rats, pubmed-meshheading:16865673-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16865673-Sequence Analysis, Protein, pubmed-meshheading:16865673-Stem Cells, pubmed-meshheading:16865673-Tumor Cells, Cultured, pubmed-meshheading:16865673-YY1 Transcription Factor, pubmed-meshheading:16865673-Zinc Fingers
pubmed:year
2006
pubmed:articleTitle
The putative human stem cell marker, Rex-1 (Zfp42): structural classification and expression in normal human epithelial and carcinoma cell cultures.
pubmed:affiliation
Department of Pharmacology, Weill Medical College, Cornell University, New York, New York 10021, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural