Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2006-7-24
pubmed:abstractText
Diabetes mellitus (DM) is a highly prevalent complex genetic disorder. There has been a worldwide effort in the identification of susceptibility genes for DM and its complications, and the 5-10-methylenetetrahydrofolate reductase (MTHFR) and apolipoprotein-E (APOE) genes have been considered good candidate susceptibility genes to this condition. The objectives of the present study were to determine if the 677T MTHFR and epsilon2/epsilon3/epsilon4 APOE alleles are risk factors for DM and for severity of diabetic retinopathy (DR). A total of 248 individuals were studied: 107 healthy individuals and 141 diabetic patients (46 with type 1 diabetes and 95 with type 2 diabetes), who also had DR (81 with non-proliferative DR and 60 with proliferative DR). The polymorphisms were analyzed by PCR followed by digestion with restriction enzyme or the single-nucleotide primer extension method. No evidence of association between the 677TT genotype of MTHFR gene and DM [cases: TT = 10/95 (10.6%); controls: TT = 14/107 (13%)] or with severity of DR was observed [cases: TT = 5/60 (8.5%); controls: TT = 9/81 (11.1%); P > 0.05]. We also did not find evidence of an association between APOE alleles and proliferative DR (epsilon2, epsilon3 and epsilon4 in cases: 9, 76, and 15%, and in controls: 5, 88, and 12%, respectively) but the carriers of epsilon2 allele were more frequent among patients with type 2 DM and DR than in controls [cases: 15/95 (15.8%); controls: 7/107 (6.5%); P < 0.05]. Therefore, our results suggest that the epsilon2 allele/APOE might be a risk factor for diabetes in the Brazilian population.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0100-879X
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
883-8
pubmed:meshHeading
pubmed-meshheading:16862278-Aged, pubmed-meshheading:16862278-Alleles, pubmed-meshheading:16862278-Apolipoproteins E, pubmed-meshheading:16862278-Case-Control Studies, pubmed-meshheading:16862278-Diabetes Mellitus, Type 1, pubmed-meshheading:16862278-Diabetes Mellitus, Type 2, pubmed-meshheading:16862278-Diabetic Retinopathy, pubmed-meshheading:16862278-Female, pubmed-meshheading:16862278-Gene Frequency, pubmed-meshheading:16862278-Genetic Predisposition to Disease, pubmed-meshheading:16862278-Genotype, pubmed-meshheading:16862278-Humans, pubmed-meshheading:16862278-Male, pubmed-meshheading:16862278-Methylenetetrahydrofolate Reductase (NADPH2), pubmed-meshheading:16862278-Middle Aged, pubmed-meshheading:16862278-Polymerase Chain Reaction, pubmed-meshheading:16862278-Polymorphism, Genetic, pubmed-meshheading:16862278-Risk Factors, pubmed-meshheading:16862278-Severity of Illness Index
pubmed:year
2006
pubmed:articleTitle
Effect of polymorphisms of the MTHFR and APOE genes on susceptibility to diabetes and severity of diabetic retinopathy in Brazilian patients.
pubmed:affiliation
Centro de Estudos do Genoma Humano, Instituto de Biociências, USP, São Paulo, SP, Brasil.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't