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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2006-7-24
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pubmed:abstractText |
Cell-mediated immunity plays an important role in immunity to the pathogenic fungus Cryptococcus neoformans. However, the antigen specificity of the T-cell response to C. neoformans remains largely unknown. In this study, we used two approaches to determine the antigen specificity of the T-cell response to C. neoformans. We report here that a diverse T-cell receptor (TCR) Vbeta repertoire was maintained throughout the primary response to pulmonary C. neoformans infection in immunocompetent mice. CD4+ T-cell deficiency resulted in relative expansion of all CD8+ T-cell subsets. During a secondary immune response, preferential usage of a TCR Vbeta subset in CD4+ T cells occurred in single individuals, but the preferences were "private" and not shared between individuals. Both CD4+ and CD8+ T cells from the secondary lymphoid tissues of immunized mice proliferated in response to a variety of C. neoformans antigens, including heat-killed whole C. neoformans, culture filtrate antigen, C. neoformans lysate, and purified cryptococcal mannoprotein. CD4+ and CD8+ T cells from the secondary lymphoid tissues of mice undergoing a primary response to C. neoformans proliferated in response to C. neoformans lysate. In response to stimulation with C. neoformans lysate, lung CD4+ and CD8+ T cells produced the effector cytokines tumor necrosis factor alpha and gamma interferon. These results demonstrate that a diverse T-cell response is generated in response to pulmonary C. neoformans infection.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-10358765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-11006012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-11035718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-11160354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-11254721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-11490008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-11884457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-12421963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-12705857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-15037737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-15710651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-15944298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-1672543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-7543591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-7612231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-7689611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-8064237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-8717525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-8890243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-9030866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-9415026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-9497484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-9653084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-9727067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861640-9916111
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4538-48
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:16861640-Animals,
pubmed-meshheading:16861640-CD4-Positive T-Lymphocytes,
pubmed-meshheading:16861640-CD8-Positive T-Lymphocytes,
pubmed-meshheading:16861640-Cryptococcosis,
pubmed-meshheading:16861640-Cryptococcus neoformans,
pubmed-meshheading:16861640-Female,
pubmed-meshheading:16861640-Interferon-gamma,
pubmed-meshheading:16861640-Lung,
pubmed-meshheading:16861640-Lung Diseases, Fungal,
pubmed-meshheading:16861640-Lymphocyte Activation,
pubmed-meshheading:16861640-Mice,
pubmed-meshheading:16861640-Mice, Inbred CBA,
pubmed-meshheading:16861640-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:16861640-Spleen,
pubmed-meshheading:16861640-T-Lymphocyte Subsets,
pubmed-meshheading:16861640-Tumor Necrosis Factor-alpha
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pubmed:year |
2006
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pubmed:articleTitle |
Diversity of the T-cell response to pulmonary Cryptococcus neoformans infection.
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pubmed:affiliation |
Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, 6301 MSRB III, University of Michigan, Ann Arbor, Michigan 48109-0642, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, N.I.H., Extramural
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