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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2006-8-31
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pubmed:abstractText |
The genome of the hyperthermophilic archaeon Sulfolobus solfataricus contains dozens of small C/D-box sRNAs that use a complementary guide sequence to target 2'-O-ribose methylation to specific locations within ribosomal and transfer RNAs. The sRNAs are approximately 50-60 nucleotides in length and contain two RNA structural kink-turn (K-turn) motifs that are required for assembly with ribosomal protein L7Ae, Nop5, and fibrillarin to form an active ribonucleoprotein (RNP) particle. The complex catalyzes guide-directed methylation to target RNAs. Earlier work in our laboratory has characterized the assembly pathway and methylation reaction using the model sR1 sRNA from Sulfolobus acidocaldarius. This sRNA contains only one antisense region situated adjacent to the D-box, and methylation is directed to position U52 in 16S rRNA. Here we have investigated through RNA mutagenesis, the relationship between the sR1 structure and methylation-guide function. We show that although full activity of the guide requires intact C/D and C'/D' K-turn motifs, each structure plays a distinct role in the methylation reaction. The C/D motif is directly implicated in the methylation function, whereas the C'/D' element appears to play an indirect structural role by facilitating the correct folding of the RNA. Our results suggest that L7Ae facilitates the folding of the K-turn motifs (chaperone function) and, in addition, is required for methylation activity in the presence of Nop5 and Fib.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861619-10024446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16861619-10395551,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/16861619-9255067
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Archaeal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Archaeal,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Guide,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 16S,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ribosomal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/fibrillarin
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1355-8382
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1708-20
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:16861619-Archaeal Proteins,
pubmed-meshheading:16861619-Base Sequence,
pubmed-meshheading:16861619-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:16861619-Methylation,
pubmed-meshheading:16861619-Molecular Sequence Data,
pubmed-meshheading:16861619-Mutagenesis, Site-Directed,
pubmed-meshheading:16861619-Nucleic Acid Conformation,
pubmed-meshheading:16861619-RNA, Archaeal,
pubmed-meshheading:16861619-RNA, Guide,
pubmed-meshheading:16861619-RNA, Ribosomal, 16S,
pubmed-meshheading:16861619-Recombinant Proteins,
pubmed-meshheading:16861619-Ribosomal Proteins,
pubmed-meshheading:16861619-Sulfolobus solfataricus
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pubmed:year |
2006
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pubmed:articleTitle |
Probing the structure and function of an archaeal C/D-box methylation guide sRNA.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada. arina@interchange.ubc.ca
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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