Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-3-16
pubmed:abstractText
Hepatic nuclear factor 1 (HNF1) is a highly diverged homeoprotein that is crucial for transcription of many liver-specific genes including albumin. In particular, a minimal promoter, consisting of an HNF1-binding-site and a TATA box, is highly active only in hepatoma cell lines. The expression of the HNF1 and albumin genes has been examined in mouse embryos by in situ hybridization. At 10.5 days of gestation, the HNF1 mRNA was detected in both the hepatic primordia and visceral endoderm of the yolk sac whereas the albumin transcript was present only in the nascent liver. At later stages of development, HNF1 was detected in liver, in the epithelial cells of most of the digestive tract and in the cortex of the kidney, whereas albumin was again found only in the liver. The presence of HNF1 protein in adult kidney was demonstrated by immunodetection in gel-retardation assays and western blot analysis. These experiments show that, even though the HNF1 homeo-protein is essential for expression of many liver-specific genes, it cannot, by itself, force high expression levels of these genes, in non-hepatic tissues.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Albumins, http://linkedlifedata.com/resource/pubmed/chemical/Antisense Elements (Genetics), http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1, http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1-alpha, http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1-beta, http://linkedlifedata.com/resource/pubmed/chemical/Hnf1a protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Hnf1b protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
113
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
589-99
pubmed:dateRevised
2009-11-24
pubmed:meshHeading
pubmed-meshheading:1685988-Albumins, pubmed-meshheading:1685988-Animals, pubmed-meshheading:1685988-Antisense Elements (Genetics), pubmed-meshheading:1685988-DNA-Binding Proteins, pubmed-meshheading:1685988-Embryo, Mammalian, pubmed-meshheading:1685988-Gene Expression, pubmed-meshheading:1685988-Genes, Homeobox, pubmed-meshheading:1685988-Hepatocyte Nuclear Factor 1, pubmed-meshheading:1685988-Hepatocyte Nuclear Factor 1-alpha, pubmed-meshheading:1685988-Hepatocyte Nuclear Factor 1-beta, pubmed-meshheading:1685988-Kidney, pubmed-meshheading:1685988-Liver, pubmed-meshheading:1685988-Mice, pubmed-meshheading:1685988-Mice, Inbred Strains, pubmed-meshheading:1685988-Nuclear Proteins, pubmed-meshheading:1685988-Nucleic Acid Hybridization, pubmed-meshheading:1685988-RNA, Messenger, pubmed-meshheading:1685988-Transcription, Genetic, pubmed-meshheading:1685988-Transcription Factors
pubmed:year
1991
pubmed:articleTitle
Hepatic nuclear factor 1 (HNF1) shows a wider distribution than products of its known target genes in developing mouse.
pubmed:affiliation
UA 1149 du CNRS, Département des Biotechnologies, Institut Pasteur, Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't