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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1992-3-9
|
| pubmed:abstractText |
Synthetic oligonucleotide primers were used in a polymerase chain reaction (PCR) protocol to target a specific sequence in the gene coding for the A subunit of Escherichia coli verotoxin (VTe-variant, VTev). This PCR protocol permits the VTe-variant target sequence to be distinguished from closely related sequences in the same coding regions for type 1, type 2, and type 2 variant E. coli verotoxins. This procedure will be a valuable adjunct to other DNA amplification techniques currently being used for molecular epidemiological studies of verotoxigenic E. coli.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0378-1097
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
68
|
| pubmed:geneSymbol |
VT
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
227-30
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1685720-Bacterial Toxins,
pubmed-meshheading:1685720-Base Sequence,
pubmed-meshheading:1685720-Deoxyribonucleotides,
pubmed-meshheading:1685720-Escherichia coli,
pubmed-meshheading:1685720-Molecular Sequence Data,
pubmed-meshheading:1685720-Polymerase Chain Reaction,
pubmed-meshheading:1685720-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:1685720-Shiga Toxin 1
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Amplification by the polymerase chain reaction of a specific target sequence in the gene coding for Escherichia coli verotoxin (VTe variant).
|
| pubmed:affiliation |
National Laboratory for Enteric Pathogens, Laboratory Centre for Disease Control, Ottawa, Ontario, Canada.
|
| pubmed:publicationType |
Journal Article
|