Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0034790,
umls-concept:C0039194,
umls-concept:C0085358,
umls-concept:C0185117,
umls-concept:C0330390,
umls-concept:C0439828,
umls-concept:C0443199,
umls-concept:C1332714,
umls-concept:C1332717,
umls-concept:C1413244,
umls-concept:C1706438,
umls-concept:C2698600,
umls-concept:C2911684
|
pubmed:issue |
3
|
pubmed:dateCreated |
1992-3-5
|
pubmed:abstractText |
Studies in transgenic and inbred strains of mice have shown that the critical molecular interactions controlling positive selection involve major histocompatibility complex (MHC), T-cell receptor (TCR), and CD4 or CD8 coreceptor molecules. Correlations have been established between MHC gene products and the percentage of CD4 or CD8 T cells that express specific variable (V) beta-gene products as part of the alpha beta heterodimer. These studies have important implications regarding potential mechanisms of HLA-linked autoimmune diseases in humans. If similar interactions are required for positive selection in humans, one would predict that the TCR repertoire expressed by mature, peripheral blood CD4 and CD8 T cells would vary. To test this hypothesis the expression of specific TCR V beta-region genes by CD4 and CD8 T cells from healthy individuals was compared using both triple-color flow cytometry and polymerase chain reaction based experimental approaches. The results show that the TCR repertoire does vary as a function of CD4 and CD8 T-cell subsets. Among unrelated individuals certain V beta genes were consistently overrepresented in the CD4 population (V beta-5.1, -6.7a, and -18); some were skewed to the CD8 population (V beta-14) while others showed variable patterns (V beta-12 and -17). Deletion of entire V beta gene families was not observed suggesting that this is a rare event in humans. Attempts to correlate the expressed TCR repertoire in humans with HLA alleles will require consideration of these differences in expression as a function of subset.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
0198-8859
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
32
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
194-202
|
pubmed:dateRevised |
2008-11-21
|
pubmed:meshHeading |
pubmed-meshheading:1685492-Antibodies, Monoclonal,
pubmed-meshheading:1685492-Base Sequence,
pubmed-meshheading:1685492-CD4-Positive T-Lymphocytes,
pubmed-meshheading:1685492-Chromatography, High Pressure Liquid,
pubmed-meshheading:1685492-DNA,
pubmed-meshheading:1685492-Flow Cytometry,
pubmed-meshheading:1685492-Gene Expression,
pubmed-meshheading:1685492-Genetic Variation,
pubmed-meshheading:1685492-Humans,
pubmed-meshheading:1685492-Molecular Sequence Data,
pubmed-meshheading:1685492-Polymerase Chain Reaction,
pubmed-meshheading:1685492-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:1685492-T-Lymphocytes, Regulatory
|
pubmed:year |
1991
|
pubmed:articleTitle |
T-cell receptor variable beta genes show differential expression in CD4 and CD8 T cells.
|
pubmed:affiliation |
Department of Veterans Affairs Medical Center, Portland, OR 97207.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
|