Linked Life Data

16849583

Source:http://linkedlifedata.com/resource/pubmed/id/16849583

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
2006-7-19
pubmed:abstractText
Prostate gland development and growth requires both androgen action and epithelial-stromal communications. In fact, androgen signaling through the androgen receptor (AR) may be important in both stromal and epithelial cells of the prostate. Because interaction of AR with the coactivator, Hic-5/ARA55, results in enhanced androgen-induced transcription, we analyzed Hic-5/ARA55 expression in prostate tissue sections from normal human donors and prostate cancer patients. In each sample, Hic-5/ARA55 expression was confined to the stromal compartment of the prostate. Furthermore, a prostate stromal cell line, WPMY-1 cells, expresses Hic-5/ARA55, which is localized both at focal adhesion complexes and within the soluble cytoplasmic compartment. The ability of Hic-5/ARA55 to shuttle between the nuclear and cytoplasmic compartments was revealed on inhibition of nuclear export with leptomycin B. Small interfering RNA ablation experiments established endogenous Hic-5/ARA55 as a coactivator for both viral and endogenous cellular AR-regulated genes. Finally, the mechanism of Hic-5/ARA55 coactivator activity in WPMY-1 cells was revealed by chromatin immunoprecipitation analysis that showed its androgen-dependent recruitment to the promoter of the stromal androgen-responsive keratinocyte growth factor gene. These data provide the first demonstration of a stromal-specific AR coactivator that has an effect on an androgen-regulated growth factor that is essential for stromal/epithelial cell communication in the prostate.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7326-33
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:16849583-Amino Acid Sequence, pubmed-meshheading:16849583-Animals, pubmed-meshheading:16849583-Cell Line, pubmed-meshheading:16849583-Cell Nucleus, pubmed-meshheading:16849583-Cytoplasm, pubmed-meshheading:16849583-Cytoskeletal Proteins, pubmed-meshheading:16849583-DNA-Binding Proteins, pubmed-meshheading:16849583-Fibroblast Growth Factor 7, pubmed-meshheading:16849583-Humans, pubmed-meshheading:16849583-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:16849583-LIM Domain Proteins, pubmed-meshheading:16849583-Male, pubmed-meshheading:16849583-Mice, pubmed-meshheading:16849583-Molecular Sequence Data, pubmed-meshheading:16849583-Promoter Regions, Genetic, pubmed-meshheading:16849583-Prostate, pubmed-meshheading:16849583-Prostatic Neoplasms, pubmed-meshheading:16849583-Receptors, Androgen, pubmed-meshheading:16849583-Sequence Alignment, pubmed-meshheading:16849583-Stromal Cells, pubmed-meshheading:16849583-Transcriptional Activation
pubmed:year
2006
pubmed:articleTitle
Hic-5/ARA55, a LIM domain-containing nuclear receptor coactivator expressed in prostate stromal cells.
pubmed:affiliation
Department of Cell Biology and Physiology and Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural