Linked Life Data

16847552

Source:http://linkedlifedata.com/resource/pubmed/id/16847552

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2006-11-23
pubmed:abstractText
Sapovirus (SV) causes gastroenteritis in humans and comprises genetically divergent viruses. A nested reverse transcription-polymerase chain reaction (RT-PCR) targeting the capsid-protein-coding region was developed using universal and genogroup-specific primer sets. The universal primers were capable of detecting human SV genogroups I, II, IV and V. Genetic analysis of the amplified products enabled us to phylogenetically determine the genotypes of the viruses. In addition, genogroup-specific primers that amplified different lengths of the amplicon depending on the genogroup were developed. These genogroup-specific primers were also used as inner primers for the nested PCR. These two simple RT-PCR methods are powerful tools for both detection and epidemiological studies of human SV.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0304-8608
pubmed:author
pubmed:issnType
Print
pubmed:volume
151
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2503-9
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
The detection of human sapoviruses with universal and genogroup-specific primers.
pubmed:affiliation
Division of Virology, Chiba Prefectural Institute of Public Health, Chuo-ku, Chiba-shi, Chiba, Japan. m.okd4@mc.pref.chiba.lg.jp
pubmed:publicationType
Journal Article