Linked Life Data

16840777

Source:http://linkedlifedata.com/resource/pubmed/id/16840777

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
36
pubmed:dateCreated
2006-9-4
pubmed:abstractText
We studied the mechanisms of antigen presentation of CD4 T cell epitopes of the capsular Caf1 antigen of Yersinia pestis using murine bone marrow macrophages as antigen presenting cells and T cell hybridomas specific for major histocompatibility complex (MHC) class II-restricted epitopes distributed throughout the Caf1 sequence. The data revealed diversity in the pathways used and the degrees of antigen processing required depending on the structural context of epitopes within the Caf1 molecule. Two epitopes in the carboxyl-terminal globular domain were presented by newly synthesized MHC class II after low pH-dependent lysosomal processing, whereas an epitope located in a flexible amino-terminal strand was presented by mature MHC class II independent of low pH and with no detectable requirement for proteolytic processing. A fourth epitope located between the two regions of Caf1 showed intermediate behavior. The data are consistent with progressive unfolding and cleavage of rCaf1 from the amino terminus as it traverses the endosomal pathway, the availability of epitopes determining which pool of MHC class II is preferentially loaded. The Caf1 capsular protein is a component of second generation plague vaccines and an understanding of the mechanisms and pathways of MHC class II-restricted presentation of multiple epitopes from this candidate vaccine antigen should inform the choice of delivery systems and adjuvants that target vaccines successfully to appropriate intracellular locations to induce protective immune responses against as wide a T cell repertoire as possible.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
281
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
26129-35
pubmed:meshHeading
pubmed-meshheading:16840777-Animals, pubmed-meshheading:16840777-Antigen Presentation, pubmed-meshheading:16840777-Antigens, Bacterial, pubmed-meshheading:16840777-Bacterial Capsules, pubmed-meshheading:16840777-Bacterial Proteins, pubmed-meshheading:16840777-Endosomes, pubmed-meshheading:16840777-Epitopes, pubmed-meshheading:16840777-Genes, MHC Class II, pubmed-meshheading:16840777-Golgi Apparatus, pubmed-meshheading:16840777-HLA-D Antigens, pubmed-meshheading:16840777-Humans, pubmed-meshheading:16840777-Hybridomas, pubmed-meshheading:16840777-Macrophages, pubmed-meshheading:16840777-Mice, pubmed-meshheading:16840777-Mice, Inbred BALB C, pubmed-meshheading:16840777-Models, Molecular, pubmed-meshheading:16840777-Plague, pubmed-meshheading:16840777-Plague Vaccine, pubmed-meshheading:16840777-Protein Denaturation, pubmed-meshheading:16840777-Protein Structure, Tertiary, pubmed-meshheading:16840777-Rats, pubmed-meshheading:16840777-Recombinant Proteins, pubmed-meshheading:16840777-T-Lymphocytes, pubmed-meshheading:16840777-Yersinia pestis
pubmed:year
2006
pubmed:articleTitle
Sequential proteolytic processing of the capsular Caf1 antigen of Yersinia pestis for major histocompatibility complex class II-restricted presentation to T lymphocytes.
pubmed:affiliation
Musculoskeletal Research Group, Institute of Cellular Medicine, University of Newcastle, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't