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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
30
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pubmed:dateCreated |
2006-7-26
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pubmed:abstractText |
Since its introduction a decade ago, whole-genome shotgun sequencing (WGS) has been the main approach for producing cost-effective and high-quality genome sequence data. Until now, the Sanger sequencing technology that has served as a platform for WGS has not been truly challenged by emerging technologies. The recent introduction of the pyrosequencing-based 454 sequencing platform (454 Life Sciences, Branford, CT) offers a very promising sequencing technology alternative for incorporation in WGS. In this study, we evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730xl Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0027-8424
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pubmed:author |
pubmed-author:BusamDanaD,
pubmed-author:FeldblyumTamaraT,
pubmed-author:FerrieraSteveS,
pubmed-author:FrazierMarvinM,
pubmed-author:FriedmanRobertR,
pubmed-author:GoldbergSusanne M DSM,
pubmed-author:HalpernAaronA,
pubmed-author:JohnsonJustinJ,
pubmed-author:KhouriHodaH,
pubmed-author:KravitzSaul ASA,
pubmed-author:LauroFederico MFM,
pubmed-author:LiKelvinK,
pubmed-author:RogersYu-HuiYH,
pubmed-author:StrausbergRobertR,
pubmed-author:SuttonGrangerG,
pubmed-author:TallonLukeL,
pubmed-author:ThomasTorstenT,
pubmed-author:VenterEliE,
pubmed-author:VenterJ CraigJC
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|
pubmed:issnType |
Print
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|
pubmed:day |
25
|
|
pubmed:volume |
103
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pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
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pubmed:pagination |
11240-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
|
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pubmed:year |
2006
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pubmed:articleTitle |
A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes.
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pubmed:affiliation |
J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA. sgoldberg@venterinstitute.org
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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