Linked Life Data

16839727

Source:http://linkedlifedata.com/resource/pubmed/id/16839727

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
2007-2-23
pubmed:abstractText
The aim of this study was to determine if ancient hair shafts could be suitable for nuclear DNA analysis and to develop an efficient and straightforward protocol for DNA extraction and STR typing of ancient specimens. The developed method was validated on modern and forensic samples and then successfully applied on ancient hairs collected from Siberian mummies dating from the 16th to the early 19th centuries. In parallel extractions including or excluding a washing step were performed at least two times for each sample in order to evaluate the influence on the quantity of nuclear DNA yielded and on the typing efficiency. Twelve ancient individuals were analyzed through our approach and full and reliable profiles were obtained for four of them. These profiles were validated by comparison with those obtained from bone and teeth DNA extracted from the same ancient specimens. The present study demonstrates that the washing step cannot be considered as deleterious for DNA retrieval since the same results were obtained by the two approaches. This finding challenges the hypothesis that recoverable nuclear DNA is only found on the outer surface of hair shafts and provides evidence that nuclear DNA can be successfully extracted from ancient hair shafts. The method described here constitutes a promising way for non-invasive investigations in ancient DNA analysis for precious or historical samples as well as forensic casework analyses.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0379-0738
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
166
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
218-29
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
STR typing of ancient DNA extracted from hair shafts of Siberian mummies.
pubmed:affiliation
Laboratory of Molecular Anthropology, Institute of Legal Medicine of Strasbourg, 11 rue Humann, 67085 Strasbourg Cedex, France. sylvain.amory@iml-ulp.u-strabg.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't