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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4-6
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pubmed:dateCreated |
2006-7-14
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pubmed:abstractText |
We examined the combinatorial action of RNase P and tRNase ZL-mediated specific inhibition of HIV-1 in cultured cells. We designed two short extra guide sequences (sEGS) that specifically recognize the tat and vifregions of HIV-1 mRNA and mediate the subsequent cleavage of hybridized mRNA by the RNase P and tRNase ZL components. We constructed an RNase P and tRNase ZL-associated vif and tat sEGS expression vector; which used the RNA-polymerase III dependent U6 promoter, as an expression cassette for EGS. Together, the RNase P and tRNase ZL-associated sEGS molecules allow more efficient suppression of HIV-1 mRNA production when separately applied. The possibilities offered by the vector to encode sEGS will provide a powerful tool for gene therapy.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Endoribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tat,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, vif,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Catalytic,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonuclease P,
http://linkedlifedata.com/resource/pubmed/chemical/tat Gene Products, Human...,
http://linkedlifedata.com/resource/pubmed/chemical/vif Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:issn |
1525-7770
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
427-37
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:16838836-Animals,
pubmed-meshheading:16838836-Base Sequence,
pubmed-meshheading:16838836-COS Cells,
pubmed-meshheading:16838836-Cercopithecus aethiops,
pubmed-meshheading:16838836-Endoribonucleases,
pubmed-meshheading:16838836-Gene Products, tat,
pubmed-meshheading:16838836-Gene Products, vif,
pubmed-meshheading:16838836-Gene Therapy,
pubmed-meshheading:16838836-HIV-1,
pubmed-meshheading:16838836-Molecular Sequence Data,
pubmed-meshheading:16838836-Nucleic Acid Conformation,
pubmed-meshheading:16838836-RNA, Catalytic,
pubmed-meshheading:16838836-RNA, Small Interfering,
pubmed-meshheading:16838836-RNA, Viral,
pubmed-meshheading:16838836-Ribonuclease P,
pubmed-meshheading:16838836-Virus Replication,
pubmed-meshheading:16838836-tat Gene Products, Human Immunodeficiency Virus,
pubmed-meshheading:16838836-vif Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2006
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pubmed:articleTitle |
Suppression of HIV-1 replication by a combination of endonucleolytic ribozymes (RNase P and tRNnase ZL).
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pubmed:affiliation |
Department of Life and Environmental Science, Faculty of Engineering, Chiba Institute of Technology, Narashino, Chiba, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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