Linked Life Data

16837724

Source:http://linkedlifedata.com/resource/pubmed/id/16837724

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-7-13
pubmed:abstractText
This study investigated the association between Plasmodium falciparum chloroquine resistance transporter (pfcrt) T76 and P. falciparum multidrug resistance gene 1 (pfmdr1) Y86 alleles and in vivo amodiaquine (AQ) resistance, as well as the clearance of parasites harboring these two alleles in children treated with AQ in southwest Nigeria. One hundred one children with acute uncomplicated P. falciparum malaria infections were treated with the standard dosage of AQ and followed-up for 28 days. Blood samples were collected on filter paper samples at enrollment and during follow-up for identification of parasite genotypes and pfcrt and pfmdr1 mutations using polymerase chain reaction and restriction fragment length polymorphism approaches. Parasitologic assessment of response to treatment showed that 87% and 13% (RI) of patients were cured and failed treatment, respectively. Although infections in patients were polyclonal (as determined by merozoite surface protein 2 genotyping), the presence of both mutants pfcrtT76 and pfmdr1Y86 alleles in parasites is associated with in vivo AQ resistance (odds ratio = 7.58, 95% confidence interval = 1.58-36.25, P = 0.006) and is selected by the drug in children who failed AQ treatment. Treatment failure with the combination of mutant pfcrtT76 and pfmdr1Y86 alleles as well as the ability of patients to clear these resistant parasites is dependent on age, suggesting a critical role of host immunity in clearing AQ-resistant P. falciparum. The combination of mutant pfcrtT76 and pfmdr1Y86 alleles may be useful markers for monitoring the development and spread of AQ resistance, when combining this drug with other antimalarials for treatment of malaria in Africa.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0002-9637
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
155-61
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:16837724-Age Factors, pubmed-meshheading:16837724-Amodiaquine, pubmed-meshheading:16837724-Animals, pubmed-meshheading:16837724-Antigens, Protozoan, pubmed-meshheading:16837724-Antimalarials, pubmed-meshheading:16837724-Child, pubmed-meshheading:16837724-Child, Preschool, pubmed-meshheading:16837724-Drug Resistance, pubmed-meshheading:16837724-Female, pubmed-meshheading:16837724-Genes, MDR, pubmed-meshheading:16837724-Genotype, pubmed-meshheading:16837724-Humans, pubmed-meshheading:16837724-Infant, pubmed-meshheading:16837724-Malaria, Falciparum, pubmed-meshheading:16837724-Male, pubmed-meshheading:16837724-Membrane Proteins, pubmed-meshheading:16837724-Membrane Transport Proteins, pubmed-meshheading:16837724-Nigeria, pubmed-meshheading:16837724-Plasmodium falciparum, pubmed-meshheading:16837724-Point Mutation, pubmed-meshheading:16837724-Protozoan Proteins
pubmed:year
2006
pubmed:articleTitle
Association between mutations in Plasmodium falciparum chloroquine resistance transporter and P. falciparum multidrug resistance 1 genes and in vivo amodiaquine resistance in P. falciparum malaria-infected children in Nigeria.
pubmed:affiliation
Malaria Research Laboratories, Institute for Advanced Medical Research and Training, College of Medicine, University of Ibadan, Ibadan, Nigeria. christianhappi@hotmail.com
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural