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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1992-1-6
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pubmed:abstractText |
Early passage bovine brain capillary endothelial cells were immortalized by transfection with the plasmid pSV3 neo. Cells from one clone, SV-BEC, expressed nuclear SV 40 large T antigen, displayed a contact-inhibited and anchorage-dependent proliferation, and a high sensitivity to the addition of exogenous basic fibroblast growth factor. SV-BEC cells are morphologically unaltered and express typical markers of endothelial cells: Factor VIII-related antigen, angiotensin-converting enzyme and Griffonia simplicifolia agglutinin binding site. Endothelium like immunoreactivity was detected in the conditioned medium from these cells. Moreover, SV-BECs present numerous intercellular tight junctions characteristic of the blood-brain barrier and possess functional beta 1- and beta 2-adrenergic receptors, as observed on isolated bovine brain capillaries.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic beta-Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Polyomavirus Transforming,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/CGP 12177,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelins,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factor 2,
http://linkedlifedata.com/resource/pubmed/chemical/Isoproterenol,
http://linkedlifedata.com/resource/pubmed/chemical/Propanolamines,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic, beta
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0883-8364
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
27A
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
771-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1683665-Adrenergic beta-Antagonists,
pubmed-meshheading:1683665-Animals,
pubmed-meshheading:1683665-Antigens, Polyomavirus Transforming,
pubmed-meshheading:1683665-Biological Markers,
pubmed-meshheading:1683665-Blood-Brain Barrier,
pubmed-meshheading:1683665-Capillaries,
pubmed-meshheading:1683665-Cattle,
pubmed-meshheading:1683665-Cell Division,
pubmed-meshheading:1683665-Cell Line, Transformed,
pubmed-meshheading:1683665-Cells, Cultured,
pubmed-meshheading:1683665-Cerebrovascular Circulation,
pubmed-meshheading:1683665-Culture Techniques,
pubmed-meshheading:1683665-Cyclic AMP,
pubmed-meshheading:1683665-Endothelins,
pubmed-meshheading:1683665-Endothelium, Vascular,
pubmed-meshheading:1683665-Fibroblast Growth Factor 2,
pubmed-meshheading:1683665-Intercellular Junctions,
pubmed-meshheading:1683665-Isoproterenol,
pubmed-meshheading:1683665-Microscopy, Electron,
pubmed-meshheading:1683665-Plasmids,
pubmed-meshheading:1683665-Propanolamines,
pubmed-meshheading:1683665-Radioimmunoassay,
pubmed-meshheading:1683665-Receptors, Adrenergic, beta,
pubmed-meshheading:1683665-Simian virus 40,
pubmed-meshheading:1683665-Transfection
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pubmed:year |
1991
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pubmed:articleTitle |
Immortalization of brain capillary endothelial cells with maintenance of structural characteristics of the blood-brain barrier endothelium.
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pubmed:affiliation |
Laboratoire d'Immuno-Pharmacologie Moléculaire, C.N.R.S., Université Paris VII, Institut Cochin de Génétique Moléculaire, (IGGM), Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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