Linked Life Data

16829613

Source:http://linkedlifedata.com/resource/pubmed/id/16829613

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2006-7-10
pubmed:abstractText
To determine suitability for national serosurveys, we compared two commercial enzyme-linked immunosorbent assays (ELISAs) for mumps antibody, Enzygnost Anti-Parotitis-Virus/IgG (which uses a whole-virus antigen) and Microimmune Mumps IgG Screen ELISA (which uses a recombinant nucleoprotein antigen), by testing 1,915 opportunistically collected sera submitted to diagnostic laboratories across Australia in 1997 to 1998. The proportion of positive results increased with age in both ELISAs but was significantly higher with the Microimmune than with the Enzygnost ELISA overall (88% versus 63%; P < 0.01) and in all age groups. However, the proportion of equivocal results was significantly higher with the Enzygnost than with the Microimmune ELISA (9% versus 4%; P < 0.01). Of the 572 sera with discrepant or equivocal results, 508 had sufficient sample remaining to perform the neutralization test (NT). A proportion with concordant results in both ELISAs were also tested by the NT. For sera with discrepant results, there was significantly better agreement between the NT and Microimmune than between the NT and Enzygnost (310/444 [70%] versus 135/348 [39%]; P < 0.01). Of 64 sera with equivocal Microimmune results, 45 (70%) were positive in the NT compared with 140 of 160 (88%) equivocal Enzygnost results (P < 0.01). Compared with the NT, the Microimmune ELISA is more sensitive (96% versus 80%) but apparently less specific (36% versus 85%) than the Enzygnost ELISA. However, this is likely to be due to the generally lower sensitivity of the NT, since the Microimmune results reflect expected seroprevalence, based on vaccine uptake in the age groups studied. We conclude that the Microimmune ELISA is a more appropriate assay than the Enzygnost ELISA for estimation of mumps seroprevalence.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16829613-10722140, http://linkedlifedata.com/resource/pubmed/commentcorrection/16829613-11219791, http://linkedlifedata.com/resource/pubmed/commentcorrection/16829613-11693507, http://linkedlifedata.com/resource/pubmed/commentcorrection/16829613-11725578, http://linkedlifedata.com/resource/pubmed/commentcorrection/16829613-12948369, http://linkedlifedata.com/resource/pubmed/commentcorrection/16829613-12948370, http://linkedlifedata.com/resource/pubmed/commentcorrection/16829613-1500523, http://linkedlifedata.com/resource/pubmed/commentcorrection/16829613-16188328, http://linkedlifedata.com/resource/pubmed/commentcorrection/16829613-2257134, http://linkedlifedata.com/resource/pubmed/commentcorrection/16829613-2906222, http://linkedlifedata.com/resource/pubmed/commentcorrection/16829613-3016017, http://linkedlifedata.com/resource/pubmed/commentcorrection/16829613-4978535, http://linkedlifedata.com/resource/pubmed/commentcorrection/16829613-6016061, http://linkedlifedata.com/resource/pubmed/commentcorrection/16829613-6361060, http://linkedlifedata.com/resource/pubmed/commentcorrection/16829613-6373967
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1556-6811
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
764-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Evaluation of two enzyme immunoassays for detection of immunoglobulin G antibodies to mumps virus.
pubmed:affiliation
Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, New South Wales 2145, Australia. job@icpmr.wsahs.nsw.gov.au
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Evaluation Studies