16828787

Source:http://linkedlifedata.com/resource/pubmed/id/16828787

Download in:

Switch to

Custom View

Named Graph Language Inference

Statements in which the resource exists as a subject.
Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0007616, umls-concept:C0007634, umls-concept:C0012497, umls-concept:C0162638, umls-concept:C0205263, umls-concept:C0439799, umls-concept:C0597198, umls-concept:C1135847, umls-concept:C1280412, umls-concept:C1280500, umls-concept:C1947906
pubmed:issue	1-2
pubmed:dateCreated	2006-9-4
pubmed:abstractText	Apoptosis is one of the most important phenomena in cell biology. Pre-apoptotic cells, defined as cells engaged in early stages of apoptosis, could be used as a cellular tool to study apoptosis pathways. The human 1547 osteosarcoma cell line and diosgenin (a plant steroid) association was selected as an in vitro cellular apoptosis model. In a previous study, using this model, we demonstrated that SdFFF monitored apoptosis induction as early as 6h after incubation. In this study, we investigated the capacity of Sedimentation Field-Flow Fractionation (SdFFF) to sort an enriched population of pre-apoptotic cells from 1547 cells incubated for 6 h with 40 microM diosgenin. In that way, two different separation devices which differed especially in channel thickness, 125 and 175 microm, were used and compared. Results showed, for the first time, that SdFFF is an effective method to obtain an enriched pre-apoptotic sub-population. These results suggest, as a new application, that SdFFF could be an included tool in the study of apoptotic mechanisms or the kinetic action of apoptotic drugs.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/9318488
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Diosgenin
pubmed:status	MEDLINE
pubmed:month	Sep
pubmed:issn	0021-9673
pubmed:author	pubmed-author:Bégaud-GrimaudGG, pubmed-author:BattaGG, pubmed-author:BeneytoutJ LJL, pubmed-author:CardotP J PPJ, pubmed-author:LégerD YDY, pubmed-author:LiagreBB
pubmed:issnType	Print
pubmed:day	22
pubmed:volume	1128
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	194-202
pubmed:dateRevised	2009-1-15
pubmed:meshHeading	pubmed-meshheading:16828787-Apoptosis, pubmed-meshheading:16828787-Cell Line, Tumor, pubmed-meshheading:16828787-Cell Separation, pubmed-meshheading:16828787-Diosgenin, pubmed-meshheading:16828787-Fractionation, Field Flow, pubmed-meshheading:16828787-Humans
pubmed:year	2006
pubmed:articleTitle	Pre-apoptotic sub-population cell sorting from diosgenin apoptosis induced 1547 cells by Sedimentation Field-Flow Fractionation. The effect of channel thickness on sorting performance.
pubmed:affiliation	Laboratoire de Chimie Analytique et Bromatologie, EA 3842, Homéostasie Cellulaire et Pathologies, Université de Limoges, Faculté de Pharmacie, 2 rue du Docteur Marcland, 87025 Limoges Cedex, France.
pubmed:publicationType	Journal Article, Comparative Study, In Vitro, Research Support, Non-U.S. Gov't