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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2006-9-14
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pubmed:abstractText |
Carbon monoxide (CO), a byproduct of heme catalysis, was shown to have potent cytoprotective and anti-inflammatory effects. In vivo recipient CO inhalation at low concentrations prevented ischemia/reperfusion (I/R) injury associated with small intestinal transplantation (SITx). This study examined whether ex vivo delivery of CO in University of Wisconsin (UW) solution could ameliorate intestinal I/R injury. Orthotopic syngenic SITx was performed in Lewis rats after 6 h cold preservation in control UW or UW that was bubbled with CO gas (0.1-5%) (CO-UW). Recipient survival with intestinal grafts preserved in 5%, but not 0.1%, CO-UW improved to 86.7% (13/15) from 53% (9/17) with control UW. At 3 h after SITx, grafts stored in 5% CO-UW showed improved intestinal barrier function, less mucosal denudation and reduced inflammatory mediator upregulation compared to those in control UW. Preservation in CO-UW associated with reduced vascular resistance (end preservation), increased graft cyclic guanosine monophosphate levels (1 h), and improved graft blood flow (1 h). Protective effects of CO-UW were reversed by ODQ, an inhibitor of soluble guanylyl cyclase. In vitro culture experiment also showed better preservation of vascular endothelial cells with CO-UW. The study suggests that ex vivo CO delivery into UW solution would be a simple and innovative therapeutic strategy to prevent transplant-induced I/R injury.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1600-6135
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pubmed:author |
pubmed-author:ChoiA M KAM,
pubmed-author:FinkM PMP,
pubmed-author:HaradaTT,
pubmed-author:IkedaAA,
pubmed-author:KohmotoJJ,
pubmed-author:MoritaKK,
pubmed-author:MuraseNN,
pubmed-author:NakasMM,
pubmed-author:NalesnikM AMA,
pubmed-author:StolzD BDB,
pubmed-author:TakahashiTT,
pubmed-author:TomiyamaKK,
pubmed-author:ToyokawaHH,
pubmed-author:TsunoYY,
pubmed-author:YangRR
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2243-55
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:16827783-Adenosine,
pubmed-meshheading:16827783-Allopurinol,
pubmed-meshheading:16827783-Animals,
pubmed-meshheading:16827783-Antimetabolites,
pubmed-meshheading:16827783-Carbon Monoxide,
pubmed-meshheading:16827783-Disease Models, Animal,
pubmed-meshheading:16827783-Glutathione,
pubmed-meshheading:16827783-Graft Survival,
pubmed-meshheading:16827783-Insulin,
pubmed-meshheading:16827783-Intestinal Mucosa,
pubmed-meshheading:16827783-Intestine, Small,
pubmed-meshheading:16827783-Male,
pubmed-meshheading:16827783-Microscopy, Electron, Transmission,
pubmed-meshheading:16827783-Organ Preservation,
pubmed-meshheading:16827783-Organ Preservation Solutions,
pubmed-meshheading:16827783-Organ Transplantation,
pubmed-meshheading:16827783-Raffinose,
pubmed-meshheading:16827783-Rats,
pubmed-meshheading:16827783-Rats, Inbred Lew,
pubmed-meshheading:16827783-Reperfusion Injury,
pubmed-meshheading:16827783-Treatment Outcome
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pubmed:year |
2006
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pubmed:articleTitle |
Ex vivo application of carbon monoxide in University of Wisconsin solution to prevent intestinal cold ischemia/reperfusion injury.
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pubmed:affiliation |
Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pennsylvania, USA. anakao@imap.pitt.edu
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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