Source:http://linkedlifedata.com/resource/pubmed/id/16826163
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
2006-8-17
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| pubmed:abstractText |
Immunization with inactivated autoreactive T cells may induce idiotype anti-idiotypic reactions to deplete autoreactive T cells, which are involved in autoimmune diseases. However, it is unknown whether attenuated activated healthy autologous T-cell immunization could increase anti-tumor immune responses. To this end, C57Bl/6 mice were immunized with attenuated activated autologous T cells. The splenocytes from immunized mice showed a higher proliferative ability than that from naive mice. The special phenotype analysis showed that there were more CD8+ T cells and CD62L+ T cells in immunized mice after 24 h of culture with 10% fetal calf serum complete medium in vitro (P<0.01). These results demonstrated that this immunization may activate T cells in vivo. Furthermore, the splenocytes from immunized mice revealed resistance to activation-induced cell death (AICD) in vitro. To further study the relative genes that are responsible for the higher proliferation and resistance to AICD, the expression of Fas/Fas ligand (FasL) and GADD45b was measured by real-time PCR. The results indicated that GADD45beta transcription was higher in the splenocytes from immunized mice than that in the naive mice. In addition, the Fas expression showed a parallel higher, but FasL did not change obviously. To investigate the biologic functions induced by immunization in vivo, a tumor model was established by EL-4 tumor cell inoculation in C57/Bl mice. Mice receiving autologous T-cell immunization had significantly inhibited tumor growth in vivo (P<0.01). This study implicated that immunization with attenuated activated autologous T cells enhances anti-tumor immune responses that participate in tumor growth inhibition.
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| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD95,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Fas Ligand Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Gadd45b protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
1748-7838
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
16
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
702-12
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| pubmed:meshHeading |
pubmed-meshheading:16826163-Animals,
pubmed-meshheading:16826163-Antigens, CD95,
pubmed-meshheading:16826163-Antigens, Differentiation,
pubmed-meshheading:16826163-Antineoplastic Agents,
pubmed-meshheading:16826163-Cell Death,
pubmed-meshheading:16826163-Cell Proliferation,
pubmed-meshheading:16826163-Fas Ligand Protein,
pubmed-meshheading:16826163-Female,
pubmed-meshheading:16826163-Humans,
pubmed-meshheading:16826163-Immunization,
pubmed-meshheading:16826163-Interleukin-12,
pubmed-meshheading:16826163-Killer Cells, Natural,
pubmed-meshheading:16826163-Lymphocyte Activation,
pubmed-meshheading:16826163-Mice,
pubmed-meshheading:16826163-Mice, Inbred C57BL,
pubmed-meshheading:16826163-Neoplasms,
pubmed-meshheading:16826163-T-Lymphocytes,
pubmed-meshheading:16826163-Transplantation, Autologous,
pubmed-meshheading:16826163-Up-Regulation
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| pubmed:year |
2006
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| pubmed:articleTitle |
Immunization with autologous T cells enhances in vivo anti-tumor immune responses accompanied by up-regulation of GADD45beta.
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| pubmed:affiliation |
Shanghai Jiao Tong University School of Medicine, Shanghai, China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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