Linked Life Data

1682345

Source:http://linkedlifedata.com/resource/pubmed/id/1682345

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1991-12-13
pubmed:abstractText
Cell-free HIV RNA in plasma was detected and quantitated after antiviral therapy by the polymerase chain reaction. RNA was extracted from plasma, reverse transcribed to cDNA, amplified by polymerase chain reaction, and quantitated by absorbance based on an enzyme-linked affinity assay. 72 HIV antibody-positive subjects had one plasma sample taken. 39 who were not receiving antiretroviral therapy at the time had a mean plasma HIV RNA copy number of 690 +/- 360 (mean +/- SEM) per 200 microliters of plasma, while 33 subjects who had been receiving zidovudine therapy for a minimum of 3 mo had a mean copy number of 134 +/- 219 (P less than 0.05). 27 additional HIV antibody-positive patients had two plasma samples taken before and 1 mo after initiating dideoxynucleoside therapy. Plasma HIV RNA copy number fell from 540 +/- 175 to 77 +/- 35 (P less than 0.05). Finally, nine of these subjects had two baseline samples obtained before initiating therapy and two posttreatment samples 1 and 2 mo after therapy was begun. Mean plasma RNA copy number declined from 794 +/- 274 to less than 40 (below the lower limit of sensitivity) after 1 mo of therapy, with suppression maintained after 2 mo of therapy. These results suggest that gene amplification can be used to detect and quantitate changes in plasma HIV RNA after dideoxynucleoside therapy. Plasma HIV polymerase chain reaction may be a more sensitive marker to monitor antiviral therapy, particularly in asymptomatic patients where measurement of p24 antigen or quantitative plasma cultures are negative.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1682345-1713916, http://linkedlifedata.com/resource/pubmed/commentcorrection/1682345-1823118, http://linkedlifedata.com/resource/pubmed/commentcorrection/1682345-1909709, http://linkedlifedata.com/resource/pubmed/commentcorrection/1682345-1974752, http://linkedlifedata.com/resource/pubmed/commentcorrection/1682345-1985101, http://linkedlifedata.com/resource/pubmed/commentcorrection/1682345-2010639, http://linkedlifedata.com/resource/pubmed/commentcorrection/1682345-2127682, http://linkedlifedata.com/resource/pubmed/commentcorrection/1682345-2145579, http://linkedlifedata.com/resource/pubmed/commentcorrection/1682345-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/1682345-2511447, http://linkedlifedata.com/resource/pubmed/commentcorrection/1682345-2586564, http://linkedlifedata.com/resource/pubmed/commentcorrection/1682345-2900979, http://linkedlifedata.com/resource/pubmed/commentcorrection/1682345-3053989
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9738
pubmed:author
pubmed:issnType
Print
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1755-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Reduction in plasma human immunodeficiency virus ribonucleic acid after dideoxynucleoside therapy as determined by the polymerase chain reaction.
pubmed:affiliation
Center for AIDS Research, Stanford University Medical Center, California 94305.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.