Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1991-12-13
|
| pubmed:abstractText |
A human T-cell line producing human T-cell leukemia virus type I (HTLV-I), MT-2, was injected intravenously into female F344 rats aged 5 weeks to make HTLV-I carrier rats. Antibody against HTLV-I was detected at the 5th week after MT-2 injection, and its titer reached a high plateau which continued from the 15th to the 27th week. The antibodies were against p19, p24, p28 and p53 of HTLV-I antigens from MT-2 cells. The gag, pX and LTR nucleotide sequences of HTLV-I provirus were demonstrated by using polymerase chain reaction (PCR) in the peripheral-blood mononuclear cells of 3 rats at the 44th week and 2 at the 66th to 68th week out of 8 F344 rats injected with MT-2 cells. Quantification of the HTLV-I proviral sequence revealed that 30 to 60 molecules were present in 10(5) peripheral-blood mononuclear cells, indicating that the rats were chronically infected with HTLV-I. HTLV-I-infected rats could serve as a small-animal model for studying the pathophysiological state of HTLV-I carriers and also that of HTLV-I infection on various HTLV-I-related diseases, including adult T-cell leukemia and HTLV-I-associated myelopathy.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0020-7136
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
11
|
| pubmed:volume |
49
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
N
|
| pubmed:pagination |
764-9
|
| pubmed:dateRevised |
2007-7-24
|
| pubmed:meshHeading |
pubmed-meshheading:1682281-Animals,
pubmed-meshheading:1682281-Base Sequence,
pubmed-meshheading:1682281-Carrier State,
pubmed-meshheading:1682281-Cell Line,
pubmed-meshheading:1682281-DNA, Viral,
pubmed-meshheading:1682281-Deltaretrovirus Infections,
pubmed-meshheading:1682281-Disease Models, Animal,
pubmed-meshheading:1682281-Female,
pubmed-meshheading:1682281-HTLV-I Antibodies,
pubmed-meshheading:1682281-HTLV-I Antigens,
pubmed-meshheading:1682281-Human T-lymphotropic virus 1,
pubmed-meshheading:1682281-Humans,
pubmed-meshheading:1682281-Immunoblotting,
pubmed-meshheading:1682281-Kinetics,
pubmed-meshheading:1682281-Molecular Sequence Data,
pubmed-meshheading:1682281-Polymerase Chain Reaction,
pubmed-meshheading:1682281-Rats,
pubmed-meshheading:1682281-Rats, Inbred F344,
pubmed-meshheading:1682281-T-Lymphocytes
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Infection of rats with HTLV-1: a small-animal model for HTLV-1 carriers.
|
| pubmed:affiliation |
Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|