Source:http://linkedlifedata.com/resource/pubmed/id/16820783
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2006-7-5
|
| pubmed:abstractText |
Graft-versus-host disease (GvHD) caused by alloreactive T cells within the graft is a major drawback of allogeneic BMT, but depletion of T cells leads to higher rates of relapse, opportunistic infections and graft failure. Therefore, selective removal of GvHD-inducing alloreactive T cells might be beneficial. We describe here the separation of alloresponsive T cells, based on carboxyfluorescein succimidyl ester labeling, in vitro allostimulation and FACS-sorting. In vivo effects of the separated cell populations were investigated in the context of allogeneic BMT in murine models: in vitro resting T cells were shown to survive in the allogeneic host and retain immunoreactivity against 'third-party' antigens. As demonstrated in two different transplantation models, elimination of proliferating cells significantly reduces GvHD but offers no advantages to using T-cell-depleted bone marrow alone concerning engraftment and tumor control. Transplanting T cells that proliferate in response to tumor antigens in vitro may narrow down the spectrum of antigens recognized by T cells and therefore reduce GvHD while maintaining graft-facilitating function and tumor control. Therefore, selecting tumor-reactive T cells on the basis of their proliferative response in vitro may be beneficial for the recipient, less time consuming than T-cell cloning and still reduce the extent of GvHD.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5-(6)-carboxyfluorescein diacetate...,
http://linkedlifedata.com/resource/pubmed/chemical/Fluoresceins,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RAG-1 protein,
http://linkedlifedata.com/resource/pubmed/chemical/Succinimides
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0268-3369
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
38
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
157-67
|
| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16820783-Animals,
pubmed-meshheading:16820783-B-Lymphocytes,
pubmed-meshheading:16820783-Body Weight,
pubmed-meshheading:16820783-Bone Marrow Transplantation,
pubmed-meshheading:16820783-Cell Line, Tumor,
pubmed-meshheading:16820783-Cell Proliferation,
pubmed-meshheading:16820783-Cell Separation,
pubmed-meshheading:16820783-Disease Models, Animal,
pubmed-meshheading:16820783-Fluoresceins,
pubmed-meshheading:16820783-Graft vs Host Disease,
pubmed-meshheading:16820783-Homeodomain Proteins,
pubmed-meshheading:16820783-Immunotherapy,
pubmed-meshheading:16820783-Mice,
pubmed-meshheading:16820783-Mice, Inbred BALB C,
pubmed-meshheading:16820783-Mice, Inbred C3H,
pubmed-meshheading:16820783-Mice, Inbred C57BL,
pubmed-meshheading:16820783-Mice, Inbred DBA,
pubmed-meshheading:16820783-Staining and Labeling,
pubmed-meshheading:16820783-Succinimides,
pubmed-meshheading:16820783-T-Lymphocytes,
pubmed-meshheading:16820783-Tumor Cells, Cultured
|
| pubmed:year |
2006
|
| pubmed:articleTitle |
Proliferation-based T-cell selection for immunotherapy and graft-versus-host-disease prophylaxis in the context of bone marrow transplantation.
|
| pubmed:affiliation |
II Medical Department, University of Kiel, Kiel, Germany.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, Non-U.S. Gov't
|