Linked Life Data

16820446

Source:http://linkedlifedata.com/resource/pubmed/id/16820446

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2006-7-5
pubmed:abstractText
A novel method for detecting viable and thermostable direct hemolysin (TDH)-producing or TDH-related hemolysin (TRH)-producing Vibrio parahaemolyticus in seafood was developed. The method involved (i) enrichment culture, selective for viable, motile cells penetrating a soft-agar-coated filter paper, and (ii) a multiplex PCR assay targeting both the TDH gene (tdh) and TRH gene (trh) following DNase pretreatment on the test culture to eradicate any incidental DNAs that might have been released from dead cells of tdh- or trh-positive (tdh+ trh+) strains and penetrated the agar-coated filter. A set of preliminary laboratory tests performed on 190 ml of enrichment culture that had been inoculated simultaneously with ca. 100 viable cells of a strain of tdh+ trh+ V. parahaemolyticus and dense populations of a viable strain of tdh- and trh-negative V. parahaemolyticus or Vibrio alginolyticus indicated that the method detected the presence of viable tdh+ trh+ strains. Another set of preliminary tests on 190 ml of enrichment culture that had been initially inoculated with a large number of dead cells of the tdh+ trh+ strain together with dense populations of the tdh- and trh-negative strains confirmed that the method did not yield any false-positive results. Subsequent quasi-field tests using various seafood samples (ca. 20 g), each of which was experimentally contaminated with either or both hemolysin-producing strains at an initial density of ca. 5 to 10 viable cells per gram, demonstrated that contamination could be detected within 2 working days.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16820446-10074546, http://linkedlifedata.com/resource/pubmed/commentcorrection/16820446-10379807, http://linkedlifedata.com/resource/pubmed/commentcorrection/16820446-12067606, http://linkedlifedata.com/resource/pubmed/commentcorrection/16820446-12495004, http://linkedlifedata.com/resource/pubmed/commentcorrection/16820446-12839757, http://linkedlifedata.com/resource/pubmed/commentcorrection/16820446-1480187, http://linkedlifedata.com/resource/pubmed/commentcorrection/16820446-16141674, http://linkedlifedata.com/resource/pubmed/commentcorrection/16820446-2016584, http://linkedlifedata.com/resource/pubmed/commentcorrection/16820446-3126151, http://linkedlifedata.com/resource/pubmed/commentcorrection/16820446-5391048, http://linkedlifedata.com/resource/pubmed/commentcorrection/16820446-7002028, http://linkedlifedata.com/resource/pubmed/commentcorrection/16820446-7928784, http://linkedlifedata.com/resource/pubmed/commentcorrection/16820446-8469282, http://linkedlifedata.com/resource/pubmed/commentcorrection/16820446-8509337, http://linkedlifedata.com/resource/pubmed/commentcorrection/16820446-8880318, http://linkedlifedata.com/resource/pubmed/commentcorrection/16820446-9230364, http://linkedlifedata.com/resource/pubmed/commentcorrection/16820446-9464425
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4576-82
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Soft-agar-coated filter method for early detection of viable and thermostable direct hemolysin (TDH)- or TDH-related hemolysin-producing Vibrio parahaemolyticus in seafood.
pubmed:affiliation
Department of Bioresources and Agrobiosciences, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1, Kobe City 657-8501, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies