Linked Life Data

16819288

Source:http://linkedlifedata.com/resource/pubmed/id/16819288

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-7-4
pubmed:abstractText
The inducible 70 kDa heat shock proteins (Hsp70) in mice are encoded by two almost identical genes, hsp70.1 and hsp70.3. Studies have found that only hsp70.1 is induced by hypertonic stress while both hsp70.1 and hsp70.3 genes are expressed in response to heat shock stress. It is unclear if the human counterparts, hsp70-2 and hsp70-1, are differentially regulated by heat shock and osmotic stress. This study found that only hsp70-2 was induced by hypertonic stress in human embryonic kidney epithelial cells and fibroblasts, while heat shock stress induced both hsp70-1 and hsp70-2. The human hsp70-2 promoter region contains three TonE (tonicity-responsive enhancer) sites, which were reported to play an important role in the response to hypertonicity. When the reporter plasmids containing different parts of the 5' flanking region of hsp70-2 were transfected into human embryonic kidney epithelial cells or fibroblasts, one TonE site at -135 was found to play a key role in the response to hypertonicity. The inactivation of the TonE site using site-directed mutagenesis led to the complete loss of induction by hypertonicity, which demonstrates the essential role of the TonE site. This suggests that the TonE site and the TonEBP (TonE binding protein) are the major regulators for the cellular response against high osmolarity in human kidney tissue.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1226-3613
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
295-301
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16819288-5' Flanking Region, pubmed-meshheading:16819288-Base Sequence, pubmed-meshheading:16819288-Binding Sites, pubmed-meshheading:16819288-Cell Line, pubmed-meshheading:16819288-DNA-Binding Proteins, pubmed-meshheading:16819288-Gene Expression Regulation, pubmed-meshheading:16819288-HSP70 Heat-Shock Proteins, pubmed-meshheading:16819288-Humans, pubmed-meshheading:16819288-Mutagenesis, Site-Directed, pubmed-meshheading:16819288-Point Mutation, pubmed-meshheading:16819288-Promoter Regions, Genetic, pubmed-meshheading:16819288-Protein Binding, pubmed-meshheading:16819288-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16819288-Saline Solution, Hypertonic, pubmed-meshheading:16819288-Transcription, Genetic, pubmed-meshheading:16819288-Transcription Factors
pubmed:year
2006
pubmed:articleTitle
The role of tonicity responsive enhancer sites in the transcriptional regulation of human hsp70-2 in response to hypertonic stress.
pubmed:affiliation
Department of Biochemistry, Hallym University College of Medicine, Chuncheon 200-702, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't