Linked Life Data

16817853

Source:http://linkedlifedata.com/resource/pubmed/id/16817853

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
2006-7-4
pubmed:abstractText
Urinary glycoproteins are important inhibitors of calcium oxalate crystallization and adhesion of crystals to renal cells, both of which are key mechanisms in kidney stone formation. This has been attributed to glycosylation of the proteins. In South Africa, the black population rarely form stones (incidence < 1%) compared with the white population (incidence 12-15%). A previous study involving urinary prothrombin fragment 1 from both populations demonstrated superior inhibitory activity associated with the protein from the black group. In the present study, we compared N-linked and O-linked oligosaccharides released from urinary prothrombin fragment 1 isolated from the urine of healthy and stone-forming subjects in both populations to elucidate the relationship between glycosylation and calcium oxalate stone pathogenesis. The O-glycans of both control groups and the N-glycans of the black control samples were significantly more sialylated than those of the white stone-formers. This demonstrates a possible association between low-percentage sialylation and kidney stone disease and provides a potential diagnostic method for a predisposition to kidney stones that could lead to the implementation of a preventative regimen. These results indicate that sialylated glycoforms of urinary prothrombin fragment 1 afford protection against calcium oxalate stone formation, possibly by coating the surface of calcium oxalate crystals. This provides a rationale for the established roles of urinary prothrombin fragment 1, namely reducing the potential for crystal aggregation and inhibiting crystal-cell adhesion by masking the interaction of the calcium ions on the crystal surface with the renal cell surface along the nephron.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1742-464X
pubmed:author
pubmed:issnType
Print
pubmed:volume
273
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3024-37
pubmed:dateRevised
2007-8-13
pubmed:meshHeading
pubmed-meshheading:16817853-Adult, pubmed-meshheading:16817853-African Continental Ancestry Group, pubmed-meshheading:16817853-Aged, pubmed-meshheading:16817853-Calcium Oxalate, pubmed-meshheading:16817853-Disease Susceptibility, pubmed-meshheading:16817853-European Continental Ancestry Group, pubmed-meshheading:16817853-Glycosylation, pubmed-meshheading:16817853-Humans, pubmed-meshheading:16817853-Kidney Calculi, pubmed-meshheading:16817853-Male, pubmed-meshheading:16817853-Middle Aged, pubmed-meshheading:16817853-Oligosaccharides, pubmed-meshheading:16817853-Peptide Fragments, pubmed-meshheading:16817853-Polysaccharides, pubmed-meshheading:16817853-Protein Precursors, pubmed-meshheading:16817853-Prothrombin, pubmed-meshheading:16817853-Risk, pubmed-meshheading:16817853-Risk Factors, pubmed-meshheading:16817853-Sialic Acids
pubmed:year
2006
pubmed:articleTitle
Sialylation of urinary prothrombin fragment 1 is implicated as a contributory factor in the risk of calcium oxalate kidney stone formation.
pubmed:affiliation
Department of Chemistry, University of Cape Town, South Africa.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't